中国药业2017，Vol.26Issue(8)：1-4,4.DOI:10.3969/j.issn.1006-4931.2017.08.001
沙苑子中沙苑子苷A制备方法优化研究
Preparation Method of Complanatuside A from Astragalus Complanatus
摘要
Abstract
Objective To study a simple and efficient method of extracting,separating and purifying of Complanatoside A from Astragalus Complanatus. Methods The extraction process was optimized by orthogonal test. The extract was separated by extraction,polyamide and silica gel column,and Complanatoside A was purified by methanol recrystallization. The purity was checked by spectral,TLC,HPLC methods. Results The optimum extraction conditions were as follows:the extraction time was 3 times,the ratio of liquid to material was 1 :20,the extraction time was 90 min,and the ethanol concentration was 70%. The separation and purification of Complanatoside A ac-corded with the reference was tested by spectral TLC method. The purity was more than 98. 50% tested by HPLC method. Conclusion The best extraction process has the advantages of high extraction rate and good stability,which can provide guidance for the industrial pro-duction process of Complanatoside A. The separation process has reached the technical requirements of chemical reference materials for tra-ditional Chinese medicine quality detection. The separation and purification method is simple,safe,feasible,low cost and high efficiency.关键词
沙苑子/沙苑子苷A/制备/工艺优化Key words
Astragalus Complanatus/Complanatuside A/preparation/process optimization分类
化学化工引用本文复制引用
王敏,肖顺丽,曹青云,李琦,关亮俊,张宏桂..沙苑子中沙苑子苷A制备方法优化研究[J].中国药业,2017,26(8):1-4,4.基金项目
国家自然科学基金资助项目[ 31070328 ]. （）
