中国真菌学杂志2017，Vol.12Issue(2)：65-68,4.
转化生长因子对巨噬细胞抗新生隐球菌功能的影响
Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans
摘要
Abstract
Objective To investigate the phagocytosis and killing ability of macrophage under TGF-β intervention.Methods Using TGF-β intervene phagocytosis and killing assay of activated macrophages in vitro.Phagocytic index of macrophage and the intracellular growth of Cryptococcus neoformans was observed respectively.Nitric oxide (NO) production was detected using the Griess regent kit,and then comparing the changes of NO production in macrophages under the TGF-β intervention.Results The phagocytic index with the treatment of TGF-β decreased by 43.9 ％,significantly lower than control group (P＜ 0.05).While the production of NO increased and the intracellular fungal burden significantly declined under the intervention of TGF-β (P＜0.05).Conclusion Chemotaxis and phagocytosis of activated macrophage were inhibited under the intervention of TGF-β in vitro,but the NO production increased and killing ability enhanced.关键词
新生隐球菌/转化生长因子/巨噬细胞Key words
Cryptococcus neoformans/transforming growth factor/macrophage分类
医药卫生引用本文复制引用
李颖芳,桑军军,李娟,姜伟伟,廖万清,潘炜华..转化生长因子对巨噬细胞抗新生隐球菌功能的影响[J].中国真菌学杂志,2017,12(2):65-68,4.基金项目
973项目(2013CB531606),国家自然基金(31270180) （2013CB531606）
