中国真菌学杂志2017，Vol.12Issue(2)：78-82,85,6.
芒果苷协同氟康唑抗耐药白念珠菌作用研究
Synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans
摘要
Abstract
Objective To explore the synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans.Methods The minimal inhibitory concentrations (MIC80) of 22 fluconazole-resistant Candida albicans strains by mangiferin in combination with fluconazole were determined by checkerboard microdilution assay.Time-kill curves and cell growth test were used to investigate the synergistic effect on Candida albcians growth process.The expression of CDR1,CDR2,MDR1 were measured by Real time RT-PCR.Results Mangiferin in combination with fluconazole could inhibit the growth of Candida albicans obviously (FICI＜0.5).Mangiferin and fluconazole-treated cells expressed lower level of CDR1 mRNA than the cells grown in the presence of fluconazole or mangiferin alone.Conclusions Mangiferin possessed a synergistic effect with fluconazole against fluconazole-resistant Candida albcians.关键词
耐药白念珠菌/芒果苷/氟康唑Key words
fluconazole-resistant Candida albicans/mangiferin/fluconazole分类
医药卫生引用本文复制引用
董怀怀,王元花,廖泽彬,姜远英,曹颖瑛..芒果苷协同氟康唑抗耐药白念珠菌作用研究[J].中国真菌学杂志,2017,12(2):78-82,85,6.基金项目
国家973项目(2013CB531602) （2013CB531602）
国家自然科学基金(81271798,81671991) （81271798,81671991）
