摘要
Abstract
Objective To investigate the relationship of the plasma homocysteine (Hcy) level and the polymorphism of methylenetetrahydrofolate reductase C667T (MTHFR C667T) gene with ischemic stroke (IS) in China, and analyze the correlation between Hcy and folate, VitB12. Methods The levels of plasma Hcy in 217 patients with IS and 223 matched controls were detected by enzymatic recycling method, and the MTHFR C667T genotype of the two groups by the method of polymerase chain reaction (PCR)-gene chip, then the level of plasma Hcy, the genotype frequency and the alleles distribution in two groups were compared. The levels of serum folate and VitB12 were detected by immunoassay method in 88 patients with IS and 125 controls who had not taken drugs containing folic acid or VitB12, and the correlation between folate, VitB12 and Hcy were analyzed. Results The plasma Hcy concentration in the IS group was higher than that in control group ([23.95±12.13] μmol/L vs [17.31±7.20] μmol/L, t=29.61, P<0.001); however, the frequency of homozygous and heterozygous MTHFR mutation between the two groups had no significant difference (χ 2=3.59, P=0.166), the frequency of allele C and T in the IS group were 36.6% and 63.4%, respectively, 42.8% and 57.2% in control group, there was also no significant difference in the two groups (χ 2=3.52, P=0.061). There was a significant difference in the corresponding plasma Hcy level of CC, CT, TT genotype, the plasma Hcy level of TT genotype ([25.19±12.53]μmol/L, n=162) was significantly higher than that of CT ([18.21±8.08] μmol/L,n=206) and CC ([16.65±6.90]μmol/L, n=72) genotype, and that of CT genotype was significantly higher than CC genotype (all P<0.001). The Hcy level was negatively correlated with VitB12 in the two groups (the IS: r=-0.431, the control: r=-0.507, both P<0.001) and also negatively correlated with folate(the IS: r= -0.489, the control: r=-0.446, both P<0.001). Conclusion The plasma homocysteine level of IS patients was higher than that of healthy people. The mutation of MTHFR C677T and the declined level of folic acid and VitB12 may contribute to the elevation of homocysteine level. The MTHFR gene mutation may be irrelevant to ischemic stroke.关键词
缺血性卒中/同型半胱氨酸/亚甲基四氢叶酸还原酶/叶酸/维生素B12Key words
Ischemic stroke/Homocysteine/Methylenetetrahydrofolate reductase/Folate/VitB12
