针灸临床杂志2017，Vol.33Issue(2)：45-48,封3,5.
直接灸和隔姜灸“肝俞”穴对原发性肝癌模型大鼠癌前病变期肝功能的影响
Effects of Direct Moxibustion and Ginger Moxibustion at Ganshu Point on Liver Function of Primary Liver Cancer in Rats with Precancerous Lesion
摘要
Abstract
Objective:To observe the effect of Direct moxibustion and Ginger moxibustion on liver function and liver tissue morphology of primary liver cancer in rats with precancerous lesion.Methods:A total of 55 male Wistar rats were randomly divided into a normal group (10 rats),a model group (15 rats),a Direct moxibustion group (15 rats) and a Ginger moxibustion group (15 rats).HCC models were prepared by intraperitoneal injection of diethylnitrosamine(DEN).Direct moxibustion group and Ginger moxibustion group were treated by moxa cone at Ganshu point,3 cones/acupoint,once a day,for a period of 10 weeks.At the end of modeling,glutamyltranspeptidase,aspartatetransaminase and alkaline phosphatase were checked by Elisa.HE staining was used to observe the morphological changes of liver tissues in rats.Results:After modeling,compared with those of the model group,the contents of serum GGT and AFP of the Direct moxibustion group were significantly reduced(P ＜ 0.05),and the contents of serum GGT,AST,ALP and AFP of the Ginger moxibustion group were significantly lower (P ＜ 0.05).Under the light microscopic observation of liver tissue stained with HE,the model group had tumor formation;lobular structure was completely destroyed;atypia of hyperchromatic nuclei was increased;Direct moxibustion group had a small amount of liver plate,and atypia of liver cell nuclear was low;Ginger moxibustion group had hyperchromatic nuclei deeply stained,without finding nodules.Conclusion:Direct moxibustion and Ginger moxibustion can reduce the contents of GGT,AST and ALP in serum of HCC model rats and can improve the liver function.The effect of Ginger moxibustion is superior to that of Direct moxibustion.关键词
直接灸/隔姜灸/大鼠/原发性肝癌/肝功能/甲胎蛋白Key words
Direct moxibustion/Ginger moxibustion/Rats/Primary liver cancer/Liver function/Alpha fetoprotein分类
医药卫生引用本文复制引用
王宁,谭丽,闫亚楠,成宇镇,侯中伟,熊博文,冯双双,董珍..直接灸和隔姜灸“肝俞”穴对原发性肝癌模型大鼠癌前病变期肝功能的影响[J].针灸临床杂志,2017,33(2):45-48,封3,5.基金项目
国家自然科学基金青年科学基金项目,编号:81202760. （）
