针灸临床杂志2017，Vol.33Issue(2)：49-53,封3,6.
电针肺与大肠经俞募穴改善急性胰腺炎大鼠胃肠动力减轻肺损伤的机理
Mechanism of Electro-acupuncture at Shu and Mu Acupoints of Lung and Large Intestine Meridians for Ameliorating the Gastrointestinal Motility to Reduce the Lung Injury Complicated with Acute Pancreatitis in Rats
摘要
Abstract
Objective:To explore whether electro-acupuncture (EA) at Shu and Mu acupoints of lung and large intestine meridians could ameliorate gastrointestinal motility(GM) to reduce acute lung injury (ALI) complicated with AP in rats and its mechanism when Da Cheng Qi decoction (DCQD) was used as the fundamental treatment.Methods:Forty-eight rats were randomly divided into a sham-operation group (SG),a model group (MG),a DCQD group (A),DCQD & Shu point group (B),DCQD & Mu point group (C) and DCQD & Shu and Mu points group (D),with eight rats in each group.Rats in the experimental groups were induced AP by retrograde perfusion into the biliopancreatic duct of 3.5 ％ sodium taurocholate,while group SG received normal saline instead.Two hours after injection,rats in group B,C,D were separately performed EA for three times,with the interval time of seven hours and retaining needles for forty minutes each time.Twenty-four hours after injection,rats in treatment groups were given DCQD intragastrically.Intestinal propulsion rates of all rats were measured after twelve hours of DCQD administration.Then they were sacrificed and their blood,lung and pancreas tissue samples were collected for pathological examination and CCK,VIP,MTL in blood as well as MPO and MDA were measured by ELISA.Results:Compared with Group A,CCK and MTL in group C were significantly increased and VIP was significantly decreased arid intestinal propulsion rates were also increased (P ＜ 0.05).MPO & MDA and pathology score of lung in group C were obviously lower (P ＜ 0.05);CCK in group B was obviously increased (P ＜ 0.05).MPO and pathology score of lungin group B were obviously lower (P ＜ 0.05).MTL in group D was obviously increased (P ＜ 0.05).However,other indicators in group D were not significant.Conclusion:EA at Shu and Mu acupoints of lung and large intestine meridians can increase CCK & MTL and decrease VIP to ameliorate GM and lower MPO & MDA to reduce the lung injury complicated with acute pancreatitis in rats.It shows that the Mu point is more effective.关键词
电针/肺经/大肠经/俞募穴/急性胰腺炎/胃肠动力/肺损伤Key words
Electro-acupuncture/Lung meridian/Large intestine meridian/Shu and Mu acupoints/Acute pancreatitis/Gastrointestinal motility/Lung injury分类
医药卫生引用本文复制引用
袁玲,吴俊,苏杭,刘贞艳,李思成,李娟,唐文富,万美华..电针肺与大肠经俞募穴改善急性胰腺炎大鼠胃肠动力减轻肺损伤的机理[J].针灸临床杂志,2017,33(2):49-53,封3,6.基金项目
国家自然科学基金项目,编号:81374042,81573857. （）
