浙江医学2017，Vol.39Issue(5)：327-331,335,6.DOI:10.12056/j.issn.1006-2785.2017.39.5.2016-1804
RECQL基因沉默对HCC细胞株体外增殖的抑制作用
RECQL-siRNA inhibits proliferation of human hepatocellular carcinoma cell lines in vitro
摘要
Abstract
Objective To investigate the effect of RECQL on proliferation of human hepatocellular carcinoma cell lines in vitro.Methods Hepatocellular carcinoma (HCC) BEL7404,SK-HEP-1,Hep3B and SNU-761 cells were transfected with RECQL-siRNA in vitro to silence RECQL.The expression of RECQL protein was detected with Western blot and immunohistochemistry.The viability of HCC and normal liver THLE-2 cells were examined,the expressions of apoptosis-related proteins were detected with Western blot and cell cycle distribution was observed using FACS.Results RECQL protein was positive in BEL7404,SK-HEP-1 and Hep3B cells,and negative in SNU-761 cells.RECQL-siRNA inhibited the proliferation of HCC cells significantly in the RECQL-positive expression HCC cells (P＜0.05),but had no effect on HCC SNU-761 cells and normal liver THLE-2 cells.In SK-HEP-1 cells transfected with RECQL-siRNA,the expression of cleaved caspase-3 and cleaved PARP had no significant change,but the ratio of G2/M phase cells was significantly increased (P＜0.05 or 0.01).Conclusion RECQL-siRNA can inhibit the proliferation of HCC cells with positive RECQL,indicating that RECQL may be a potential target gene for treatment of liver cancer.关键词
RECQL/人肝细胞癌/细胞凋亡/细胞周期Key words
RECQL/Human hepatocellular carcinoma/Cell apoptosis/Cell cycle引用本文复制引用
张静静,赵妍妍,许雅思,黄海琇,朱鲁程,马胜林,张仕蓉..RECQL基因沉默对HCC细胞株体外增殖的抑制作用[J].浙江医学,2017,39(5):327-331,335,6.基金项目
国家自然科学基金项目(81602671) （81602671）
浙江省自然科学基金项目(LQ17H160003) （LQ17H160003）
