浙江医学2017，Vol.39Issue(5)：340-344,后插3,6.DOI:10.12056/j.issn.1006-2785.2017.39.5.2016-1361
脂肪干细胞对角膜基质细胞增殖凋亡的调节及其机制研究
Effect of rabbit adipose-derived stem cell on proliferation and apoptosis of corneal stromal cells
摘要
Abstract
Objective To investigate the effects of rabbit adipose-derived stem cells (ADSCs) on proliferation and apoptosis of rabbit corneal stromal cells (CSCs).Methods ADSCs were isolated from subcutaneous adipose tissue in groin area of New Zealand white rabbit with the method of collagenase type Ⅰ digestion.The isolated ADSCs were cultured and passed to 3 generation and its differentiation ability was evaluated.CSCs were obtained from the corneas of New Zealand rabbits with the method of collagenase Ⅱ digestion.After initial expansion,the CSCs were cultured up to passage 2 and then prepared for experiment.The CSCs were cultured alone (control group) or co-cultured with ADSCs (indirect co-culture group).After incubation for 72 h the proliferative capacity of CSCs was evaluated by CCK-8 assay,apoptosis of CSCs was determined by flow cytometry,the expression of matrix metalloproteinases (MMPs) and collagens was detected by Western blot,and the invasion activity of corneal stromal cells was also examined in vitro.Results Compared with the control group,ADSCs significantly promoted proliferation and invasion of CSCs in indirect co-culture group (P＜0.05).The group with ADSCs showed low percentage of apoptotic cells.CSCs secreted lower levels of MMPs and higher levels of collagens in indirect co-culture group.Conclusion ADSCs can promote plasticity and EMC synthesis of corneal stromal cells,and suppress the apoptosis at the same time.It suggests that application of ADSCs may be a promising approach for corneal injury therapy.关键词
脂肪干细胞/角膜基质细胞/损伤/增殖/基质金属蛋白酶Key words
Adipose-derived stem cells/Corneal stromal cells/Injury/Proliferation/Matrix metalloproteinases引用本文复制引用
郑青青,沈江,沈婷,李秋实,洪朝阳,李文伟..脂肪干细胞对角膜基质细胞增殖凋亡的调节及其机制研究[J].浙江医学,2017,39(5):340-344,后插3,6.基金项目
浙江省科技厅重大专项(2013C03048-1) （2013C03048-1）
