浙江医学2017，Vol.39Issue(6)：416-420,5.DOI:10.12056/j.issn.1006-2785.2017.39.6.2016-2129
百里醌对兔骨关节炎模型关节软骨的保护作用及机制
Protective effects of thymoquinone on cartilage in rabbit osteoarthritis model
摘要
Abstract
Objective To evaluate the effects of intra-articular injection of thymoquinone on cartilage in rabbit osteoarthritis model.Methods Sixty New Zealand rabbits were randomly divided into treatment group,model group and control group with 20 in each group.Knee osteoarthritis model was established in thymoquinone and model groups,and sham operation was performed in control group,intraarticular injection of thymoquinone was given to treatment group.The walking distance within 6min was observed and recorded.Rabbits were sacrificed and samples of left knees were collected 9 wks after operation.Gross scores,HE staining,safranin O staining and Mankin score were evaluated.Real-time polymerase chain reactive (PCR) was performed to analysis the mRNA level of MMP-13,TIMP-1,aggrecan,collagen Ⅱ in cartilage tissue.Results Compared with model group,the thymoquinone group showed better pain related behavior scores (P＜0.05).Gross score and Mankin score showed that the cartilage lesions of thymoquinone group were less severe than that of model group.The expression of mmp-13mRNA was lower and the expressions of timp-1,aggrecan and collagen Ⅱ mRNA were higher in thymoquinone group than those in model group(P＜0.05).Conclusion Thymoquinone shows protective effects on cartilage,and can delay the disease progress and improve the pain related behavior scores in rabbit osteoarthritis model,which may be associated with down-regulation of mmp-13 expression and up-regulation of timp-1,aggrecan,collagen Ⅱ expression.关键词
百里醌/骨关节炎/动物模型Key words
Thymoquinone/Osteoarthritis/Animal model引用本文复制引用
钟甫华,江彬锋,曾宇晴,黄恺,陆建伟..百里醌对兔骨关节炎模型关节软骨的保护作用及机制[J].浙江医学,2017,39(6):416-420,5.基金项目
浙江省公益性应用研究计划项目(2015C37131) （2015C37131）
