浙江医学2017，Vol.39Issue(6)：432-434,后插3,4.DOI:10.12056/j.issn.1006-2785.2017.39.6.2016-1836
SCID小鼠永久性左侧大脑中动脉阻塞模型的制备及评价
Establishment of permanent left middle cerebral artery occlusion model in SCID mice
摘要
Abstract
Objective To establish a permanent left middle cerebral artery occlusion (L-MCAO) model in severe combined immunodeficiency (SCID) mice.Methods Thirty SCID mice were randomly divided into sham operation group and L-MCAO group with 15 in each group.The left middle cerebral artery occlusion was induced by electrocoagulation in SCID mice of L-MCAO group.The cerebral blood flow was monitored by laser Doppler,and the ratio of cerebral blood flow was calculated.The neurological deficit score was assessed 24h after operation.The animals were sacrificed two weeks after the operation;the brain tissues were stained with 2,3,5-triphenyltetrazolium chloride(TTC) and diaminobenzidine(DAB),and the percentage of cerebral infarction area was calculated.Results The ratio of cerebral blood flow in L-MCAO group was 0.47±0.55,the neurological deficit score was 1.95 ± 0.76,and the percentage of cerebral infarction area was (18.47 ± 1.92)％.While there was no cerebral infarction lesion in the sham group and model failed mice.Conclusion The permanent L-MCAO model of SCID mice has been established with high success rate,which simulates human cerebral infarction to a certain extent,and may be used for research on cerebral infarction and drug development.关键词
严重免疫缺陷/小鼠/左侧大脑中动脉闭塞术/脑血流量Key words
Severe combined immunodeficiency/Mouse/Left middle cerebral artery occlusion/Cerebral blood flow引用本文复制引用
汤倩倩,卢山,蒋敏海..SCID小鼠永久性左侧大脑中动脉阻塞模型的制备及评价[J].浙江医学,2017,39(6):432-434,后插3,4.基金项目
杭州市科技发展计划项目(20150733Q07),杭州市卫生科技计划(重点)项目(2014Z03) （20150733Q07）
