浙江医学2017，Vol.39Issue(6)：463-465,3.DOI:10.12056/j.issn.1006-2785.2017.39.6.2016-1585
细菌培养法、ELFIA和PCR检测恶性肿瘤化疗期腹泻患者艰难梭菌的比较
Comparison of bacterial culture, ELFIA and PCR for detecting clostridium difficile infection in cancer patients with diarrhea during chemotherapy
摘要
Abstract
Objective To compare bacterial culture,enzyme-linked fluorescent immunoassay (ELFIA) and polymerase chain reaction (PCR) for detecting the clostridium difficile infection (CDI) in cancer patients with diarrhea during chemotherapy.Methods Two hundred and twelve cancer patients with diarrhea during chemotherapy were enrolled in this study.Positive detection rate of Clostridium difficile was compared among fecal bacterial culture,ELFIA for CD toxin A/B and PCR for tcd A/B.The clinical features of the patients with CDI were also analyzed.Results The positive detection rates of bacteria culture,ELFIA and PCR were 12.7％,10.4％ and 31.6％,respectively,and PCR was the most sensitive one(P＜0.05).In 67 CDI patients detected by PCR,36 were males and 31 females with a mean age of 67.2 ± 10.8 years,and 58 (86.6％) were complicated with cardiovascular disease or diabetes.All patients had treatment with antibiotics and proton pump inhibitors.In all CDI patients,95.5％ cases were found positive fecal occult blood test,and the average plasma albumin was 37.92g/L and C-reactive protein was 30.25mg/L.Conclusion Among the three detection methods for clostridium difficile,PCR is the most sensitive one.For elderly cancer patients having treatment with proton pump inhibitors and broad-spectrum antibiotics and with positive fecal occult blood test,if diarrhea occurs during the chemotherapy Clostridium difficile infection should be considered.关键词
恶性肿瘤/化疗/腹泻/艰难梭菌Key words
Cancer/Chemotherapy/Diarrhea/Clostridium difficile引用本文复制引用
金利民,曹晨曦,李刚,沈宇泓,阮玲娟,衡翔,鲍轶,邵平扬,陈松劲..细菌培养法、ELFIA和PCR检测恶性肿瘤化疗期腹泻患者艰难梭菌的比较[J].浙江医学,2017,39(6):463-465,3.基金项目
浙江省卫生厅科研基金项目(2014KYA211) （2014KYA211）
