安徽农业科学2017，Vol.45Issue(11)：1-2,38,3.
烟管菌T1原生质体制备及菌丝再生条件优化
Protoplast Preparation and Hyphae Regeneration Condition Optimization of Bjerkandera adusta T1
摘要
Abstract
[Objective]To study protoplasts preparation and hyphae regeneration conditions of Bjerkandera adusta.[Method] The effect of different culture conditions (hyphae age,stabilizer and stabilizer concentration,pH,enzymatic concenfration,enzymatic hydrolysis time) on protoplasts preparation and hyphae regeneration conditions of Bjerkandera adusta was studied.[Result] The fungal mycelia cultured in the liquid PDA medium at 28 ℃ for 3 days was the most suitable for protoplast preparation.15 mg/mL soluble wall enzyme in 0.5 mol /L KCl hydrolyzing for 4 hours could generate as many as 1.18×106 protoplasts/mL.The RM1 medium was the preferred regeneration medium on which the regeneration rate was as high as 1.22%.[Conclusion] The study optimize the conditions of protoplasts preparation and regeneration,and provide theory basis for further study of molecular mechanism of Bjerkandera adusta.关键词
烟管菌T1/原生质体制备/菌丝再生/优化Key words
Bjerkandera adusta T1/Protoplast preparation/Hyphae regeneration/Optimization分类
农业科技引用本文复制引用
高雪艳,储巧玲,周凌峰,韩国民..烟管菌T1原生质体制备及菌丝再生条件优化[J].安徽农业科学,2017,45(11):1-2,38,3.基金项目
国家自然科学基金项目(51309003) （51309003）
教育部博士点基金项目(20133418120003). （20133418120003）
