安徽农业科学2017，Vol.45Issue(11)：3-6,4.
榆紫叶甲病原菌分离及生防菌的筛选与鉴定
Isolation, Screening and Identification of Bacterial Agents for Biological Control of Ambrostoma quadriimpressum
摘要
Abstract
[Objective] To explore the pathogen for the biological control of Ambrostoma quadriimpressum.[Method] Some bacteria strains were isolated from A.quadriimpressum to determine the pathogenicity of Bacillus thuringinensis to each instrar of larvae fed in the laboratory.[Result] 18 bacterial isolates were obtained from diseased adults of A.quadriimpressum,among them 12 isolates showed protein-degrading and chitin-degrading activities.5 isolates showed different pathogenicity to 1st-instar and 2nd-instar larvae of A.quadriimpressum.The larvae began to die during 3-7 d after inoculation,and the accumulative mortality rate of larvae of A.quadriimpressum was 21.2%-46.8%.The accumulative mortality rate of A.quadriimpressum larvae to YJA-03 strain and YJA-10 strain were more than 30%.YJA-03 was identified as Gram-positive bacterium,a bacteria of genus Exiguobacterium identified by morphological,physiological and biochemical characteristics and PCR amplification.YJA-10 was identified as Gram-negative bacterium,belonging to genus Glutamicibacter.[Conclusion] YJA-03 strain and YJA-10 strain can be used for the biological control of A.quadriimpressum.关键词
榆紫叶甲/生物防治/菌株分离/生理生化特性Key words
Ambrostoma quadriimopressum Motschulsky/Biological control/Strain isolation/Physiological and biochemical characteristics分类
农业科技引用本文复制引用
胡基华,陈静宇,曹旭,姜威,刘宇帅,孟利强,李晶,张淑梅..榆紫叶甲病原菌分离及生防菌的筛选与鉴定[J].安徽农业科学,2017,45(11):3-6,4.基金项目
哈尔滨市应用技术研究与开发项目(2014RFQYJ043). （2014RFQYJ043）
