安徽农业科学2017，Vol.45Issue(11)：7-10,4.
热凝胶多糖产生菌株的复合诱变选育
Mutation and Screening of Curdlan-producing Strain by Combined Mutation Methods
摘要
Abstract
[Objective] In order to improve the yield and quality of curdlan.[Method] Agrobacterium sp.714 was mutated by UV and NTG.Two high-yield strains were selected by plate spreading and shaking-flask fermentation.[Result] The crude yield of curdlan from the strain 0.6-45 and strain 0.5-50 were 26.4 and 26.5 g/L,and were 6.9 and 7.0 times higher than the crude yield of parent strain (3.8 g/L).The refined yield of curdlan from the strain 0.6-45 and strain 0.5-50 were 19.4 and 18.0 g/L.The characteristics of gel showed that the fracture strength,fracture distance,springiness,cohesiveness and resilience were higher than the sample from market,and the gel strength,hardness,gumminess and chewiness were lower than sample from market.GPC results showed the molecular weights were respectively 1.57×106 and 1.27×106 Da for curdlan from the strain 0.6-45 and 0.5-50.[Conclusion] Combined mutation improves the yield of curdlan and changes the characteristics of gel.关键词
热凝胶多糖/紫外诱变/亚硝基胍诱变/高产菌株/凝胶特性Key words
Curdlan/UV mutation/NTG mutation/High-yield strain/Gel characteristics分类
生物科学引用本文复制引用
付雅欣,雷高,方青,高红亮,杨雪霞..热凝胶多糖产生菌株的复合诱变选育[J].安徽农业科学,2017,45(11):7-10,4.基金项目
上海市自然科学基金项目(15ZR1401000). （15ZR1401000）
