北京林业大学学报2017，Vol.39Issue(4)：72-78,7.DOI:10.13332/j.1000-1522.20160181
芍药属组内组间杂交及部分后代核型分析与SSR鉴定
Intrasectional and intersectional cross breeding of Paeonia and karyotype analysis and SSR identification of some hybrids
摘要
Abstract
In order to select the proper cross combinations with high compatibility, hybridization was carried out between Paeonia lactiflora 'Zhu Sha Pan ' as female parent and 6 cultivars of Sect. Paeonia and 4 cultivars of Sect. Moutan as male parents. Meanwhile, hybrids were identified by karyotype analysis and SSR markers. The results showed that:1) Hybridization in Sect. Paeonia had a high compatibility, with an average seed setting rate of 5 seeds per flower;interspecific hybridizations of Sect. Paeonia had a poor compatibility; sectional hybridizations were incompatible, only cross combination 'Zhu Sha Pan ' ×'Dao Da Chen' seeded. 2) Karyotype analysis showed that hybrids ZC4, ZC5, ZC6 and ZC7 were triploid;Among 50 SSR primers, 7 polymorphic markers were amplified and the hybrids ZC4, ZC5, ZC6 and ZC7 contained specific bents of parents, which were true hybrids. The results suggested that interspecific hybridization between 'Zhu Sha Pan ' and 'Garden Peace ', 'Pink Teacup ', 'Cream Delight' could seed;sectional hybridization 'Zhu Sha Pan' ×'Dao Da Chen' could break the cross incompatibility. SSR markers could be applied for the early hybrid identification of Paeonia.关键词
芍药/远缘杂交/核型/SSR/杂种鉴定Key words
herbaceous peony/distant hybridization/karyotype/SSR/hybrid identification分类
农业科技引用本文复制引用
刘建鑫,杨柳慧,魏冬霞,于晓南..芍药属组内组间杂交及部分后代核型分析与SSR鉴定[J].北京林业大学学报,2017,39(4):72-78,7.基金项目
北京市教育委员会科学研究与研究生培养共建项目(BLCXY201629)、国家自然科学基金项目(31400591). （BLCXY201629）
