重庆医学2017，Vol.46Issue(13)：1743-1746,4.DOI:10.3969/j.issn.1671-8348.2017.13.005
人牙源性iPSCs3种培养底物的比较
Comparison among 3 kinds of culture substrates of odontogenic induced pluripotent stem cells
摘要
Abstract
Objective To comparatively study the characteristics of 3 kinds of culture substrates of human odontogenic induced pluripotent stem cells(iPSCs).Methods The human odontogenic iPSCs were cultured by 3 kinds of substrates:mouse embryonic fibroblasts(MEF),matrigel and recombinant human vitronectin(VTN-N).The iPSCs growth situation was compared among three groups.Results The preparation time of these 3 kinds of substrates was 14,3,1 hlespectively,and,the difference was statistically significant (P＜0.05).The iPSCs reprogramming time was (30± 1.6),(26 ± 2.1),(27 ± 1.4) d,lespectively,wht that in the MEF group significantly higer than in other two groups (P＜0.05).The reprogramming efficiencies were 0.3 ％ ± 0.03 ％,0.56 ％ ± 0.08 ％,0.7 ％ ± 0.02 ％ respectively (P＜ 0.05).Three kinds of substrate could better support iPSCs growth and make them to maintain un-differentiation status.Conclusion with no heterologous animal components,and the adrantaga of simple pleparation,oonfrollable standard and shorter gramming time is easy to prepare,the standard is controllable and the reprogramming time is shorter,which is an ideal substrate for supporting iPSCs growth.关键词
人诱导性多能干细胞/小鼠胚胎成纤维细胞/基质胶/重组人玻连蛋白Key words
human induced pluripotent stem cell/MEF/matrigel/vitronectin分类
医药卫生引用本文复制引用
谭小兵,刘佳,郭宇,徐静舒,戴青原..人牙源性iPSCs3种培养底物的比较[J].重庆医学,2017,46(13):1743-1746,4.基金项目
国家自然科学基金资助项目(81360161) （81360161）
云南省教育厅基金资助项目(2015Y153). （2015Y153）
