重庆医学2017，Vol.46Issue(13)：1753-1755,3.DOI:10.3969/j.issn.1671-8348.2017.13.008
原花青素B2保护血管内皮细胞延缓凋亡及机制研究
Study on effect and mechanism of GSPB2 for protecting vascular endothelial cell and delaying apoptosis
李强 1杨成明2
作者信息
- 1. 重庆市南川区人民医院心内科 408400
- 2. 第三军医大学野战外科研究所大坪医院心内科 400042
- 折叠
摘要
Abstract
Objective To observe the protective effect of GSPB2 for delaying the human endothelial cells apoptosis with H2O2 induced human endothelial cells apoptosis as the model and to investigate its mechanism.Methods The human endothelial cells were pre-treated by 0.5 mmol H2O2,then different concentrations of GSPB2(5.0,10.0,20.0 μmol/L) was added for protecting cells.The cellular apoptosis was detected by TUNEL method;the apoptosis related molecules,such as Caspase-3,Bax,Bcl-2,and influencing apoptosis related molecules such as PI3K,p-Akt and Akt protein expression were detected by Western bl0t;Transwell chamber was used to detect cell migration situation.Results 0.5 mmol H2O2 induced obvious increase of human endothelial cells (P＜0.01).GSPB2 could significantly alleviate the H2O2 induced apoptosis of human endothelial cells(P＜0.05).10 μmol/mL GSPB2 could be close to return to H2O2 untreated control group level.Further found that Caspase-3,Bax protein expression were up-regulaed,Bcl-2 expression was down-regulated and the expression of PI3K,p-Akt and Akt wa sup-regulated.Conclusion The different concentrations of GSPB2 has a protective effect on vascular endothelial cells induced by H2O2,moreover which manifested by the concentration and time dependence.Its mechanism is related with the related molecules PI3K,p-Akt and Akt关键词
原花青素B2/凋亡/血管内皮细胞Key words
GSPB2/apoptosis/vascular endothelial cell分类
医药卫生引用本文复制引用
李强,杨成明..原花青素B2保护血管内皮细胞延缓凋亡及机制研究[J].重庆医学,2017,46(13):1753-1755,3.
