重庆医学2017，Vol.46Issue(13)：1770-1772,3.DOI:10.3969/j.issn.1671-8348.2017.13.014
细胞免疫化学p16/Ki-67双染结合DNA倍体分析预测HSIL的研究
Study on immunocytochemistry p16/Ki-67 double staining combined with DNA ploidy analysis for analyzing and predicting cervical high grade squamous intraepithelial lesion
摘要
Abstract
Objective To investigate the diagnostic value of DNA ploidy analysis combined with immunocytochemistry p16/ki-67 double staining in cervical high grade squamous intraepithelial neoplasia(HSIL) and cervical squamous cell carcinoma(SCC).Methods A total of 73 cases of cytological tests were randomly collected.Among them,53 cases were small DNA ploidy abnormal cells and 20 cases were DNA ploidy negative.The p16/Ki-67 results were detected by immunocytochemistry double staining.With the pathological results as the golden standard,the diagnostic values of DNA ploidy analysis and DNA ploidy analysis combined with p16/Ki-67 double staining in HSIL + was contrastively analyzed by pathologic results.Results Among 20 samples of DNA ploidy negative,the p16/Ki-67 double staining results all were negative.The positive predictive value of DNA ploidy analysis for HSIL + was 34.62％.The sensitivity of DNA ploidy analysis combined with p16/Ki-67 double staining for HSIL + was 84.62％,and its specificity was 92.31％,the positive predictive value was 78.57％ and the negative predictive value was 94.74％,which were significantly higher than those of DNA ploidy analysis(P＜0.05).Conclusion p16/Ki-67 double staining can significantly im prove the prediction value of HSIL.The DNA ploidy analysis combined with p16/Ki-67 double staining is an effective method for predicting HSIL +,which is suitable for the implementation in the areas with lack of medical resources.关键词
宫颈上皮内瘤样病变/p16/Ki-67双染/细胞免疫化学/DNA倍体分析Key words
cervical intra epithelial neoplasia/p16/Ki-67 double staining/immunocytochemistry/DNA ploidy analysis分类
医药卫生引用本文复制引用
张金秋,朱萍,陆敏华,董建峰,印永祥,赵华..细胞免疫化学p16/Ki-67双染结合DNA倍体分析预测HSIL的研究[J].重庆医学,2017,46(13):1770-1772,3.基金项目
江苏省无锡市卫计委妇幼健康成果技术推广项目(FYTG2016-2) （FYTG2016-2）
江苏省无锡市卫生计生科技成果和适宜技术推广项目(T201645). （T201645）
