大连工业大学学报2017，Vol.36Issue(2)：79-83,5.
紫色杆菌素在谷氨酸棒状杆菌中异源表达
The heterologous expression of violacein in Corynebacterium glutamicum
摘要
Abstract
The feasibility of heterogenous expression of violacein based on the chassis of a Corynebacterium glutamicum that could produce L-Trp was proved.Related biosynthetic pathway genes of vioA,vioB,vioC,vioD,vioE were amplified from natural violacein producer Janthinobacterium lividum,ahead of which the element of conservative SD sequences were added,and constructed with C.glutamicum/E.coli pEC-XK99E by Golden-gate DNA assembly method.The heterologous expression of violacein was accomplished in Corynebacterium glutamicum.The violacein production of (1 243-±-207) mg/L was obtained in the optimization induction time of shaking-flask batch fermentation for 90 h.The results indicated that Corynebacterium glutamicum was an appropriate host for violacein production.关键词
紫色杆菌素/谷氨酸棒状杆菌/异源表达Key words
violacein/Corynebacterium glutamicum/heterologous expression分类
生物科学引用本文复制引用
孙宏年,毕昌昊,张春枝..紫色杆菌素在谷氨酸棒状杆菌中异源表达[J].大连工业大学学报,2017,36(2):79-83,5.基金项目
国家高技术研究发展计划项目(2015AA020202) （2015AA020202）
天津市科技支撑计划重点项目(Y5M2121111). （Y5M2121111）
