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野生艾蒿的组织培养

杨莹 吴琼 徐碧莲 刘志文

大连工业大学学报2017,Vol.36Issue(2):92-95,4.
大连工业大学学报2017,Vol.36Issue(2):92-95,4.

野生艾蒿的组织培养

The tissue culture of wild Artemisia argyi

杨莹 1吴琼 1徐碧莲 1刘志文1

作者信息

  • 1. 大连工业大学生物工程学院,辽宁大连 116034
  • 折叠

摘要

Abstract

To protect wild Artemisia argyi resources,axillary bud differentiation and rooting with the stems of Artemisia argyi as explant materials were conducted by tissue culture technique.The results showed that the explants sterilized with 0.1% HgCl2 for 6-8 min played a satisfactory sterilizing effect.6-BA and IAA were the optimum hormone for differentiation and rooting of tube seedlingsrespectively.The ideal rapid propagation medium was MS + 0.5 mg/L 6-BA + 0.5 mg/L IAA,in which the buds could grow more vigorously and the root grow more quickly,and could be proliferated by 8 to 15 times cultured for 21 days.

关键词

艾蒿/组织培养/生根/快繁

Key words

Artemisia argyi/tissue culture/rooting/rapid propagation

分类

生物科学

引用本文复制引用

杨莹,吴琼,徐碧莲,刘志文..野生艾蒿的组织培养[J].大连工业大学学报,2017,36(2):92-95,4.

基金项目

辽宁省自然科学基金项目(2015020682). (2015020682)

大连工业大学学报

OA北大核心CSTPCD

1674-1404

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