动物营养学报2017，Vol.29Issue(4)：1183-1190,8.DOI:10.3969/j.issn.1006-267x.2017.04.013
不同越冬饲料对蜜蜂中肠消化酶活性、组织发育状态以及抗氧化酶基因表达的影响
Effects of Different Overwintering Feeds on Midgut Digestive Enzyme Activities, Tissue Development Status and Antioxidant Enzyme Gene Expression of Honeybees
摘要
Abstract
The experiment was conducted to study the effects of different overwintering feeds on midgut diges-tive enzyme activities, tissue development status and antioxidant enzyme gene expression of honeybees, in or-der to provide reference for the beekeepers choose winter feed overwintering feeds. At the end of October 2015, nine overwintering colonies ( without overwintering feed reserve) of local Italian honeybees ( Apis mel-lifera L.) with equal colony population were randomly divided into 3 experimental groups ( 3 colonies per group) , and were fed honey ( honey group) , fructose corn syrup ( fructose corn syrup group) and white sugar water (white sugar :water=2:1, sucrose group) as overwintering feed from November 2 to late November when bee colonies entered into overwintering period, respectively. The experiment lasted from the end of Octo-ber 2015 to early March 2016. In order to determine the midgut digestive enzyme ( amylase, sucrase and prote-ase) activities, midgut samples were collected in early November ( before overwintering) , early January ( the middle period of overwintering) and early March ( after overwintering) , respectively. And, in early January, midgut samples were collected to determine the midgut tissue development status and the relative expression lev-els of antioxidant enzyme genes, such as superoxide dismutase 1 ( Sod1) , superoxide dismutase 2 ( Sod2) and catalase ( CAT) genes. The results showed as follows:the midgut amylase activity of fructose corn syrup group and honey group was significantly higher than that of sucrose group in early January ( P<0.05) . Different over-wintering feeds did not significantly affect the midgut sucrase activity in early January and early March ( P>0.05) . The midgut protease activity of fructose corn syrup group was significantly higher than that of sucrose group and honey group in early January (P<0.05). The midgut wall thickness of sucrose group and honey group was significantly higher than that of fructose corn syrup group ( P<0.01) , and the midgut crypt deepness of sucrose group and honey group was also significantly higher than that of fructose corn syrup group ( P<0.01) . The relative expression level of Sod1 gene from midgut of sucrose group was significantly higher than that of fructose corn syrup group and honey group in early January ( P<0.05) . In conclusion, honey can im-prove midgut digestive enzyme activities of overwintering honeybees, honey and white sugar are beneficial to the development of midgut tissue of overwintering honeybees, and white sugar can improve the midgut antioxi-dant gene expression of overwintering honeybee. Therefore, honey and white sugar as the overwintering feed for honeybee are better than fructose corn syrup.关键词
糖源类型/越冬饲料/蜜蜂/中肠消化酶/中肠组织/抗氧化酶Key words
sugar type/overwintering feed/honeybees/midgut digestive enzymes/midgut tissue/antioxidant enzymes分类
农业科技引用本文复制引用
刘春蕾,胥保华,刘振国,王颖,王红芳..不同越冬饲料对蜜蜂中肠消化酶活性、组织发育状态以及抗氧化酶基因表达的影响[J].动物营养学报,2017,29(4):1183-1190,8.基金项目
国家蜂产业技术体系建设专项资金(CARS-45) （CARS-45）
山东省农业良种工程项目"优质高产蜜蜂及蚕桑新品种培育"(2014-2016) （2014-2016）
