动物营养学报2017，Vol.29Issue(4)：1191-1197,7.DOI:10.3969/j.issn.1006-267x.2017.04.014
益生芽孢杆菌脂肽类抑菌成分的高效液相色谱-电喷雾串联质谱分析
High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry Analysis of Lipopeptides Produced by Probiotic Bacillus spp.
摘要
Abstract
The aim of this paper was to study the antibacterial effect on the intestinal bacterial and active con-stituent of Bacillus subtilis N2-10 strain with a latent probiotic capability, to lay the foundation for the healthy function and mechanism of the in-depth evaluation of the probiotic strains. The antibacterial activity of the in-gredients from culture broth was detected by the method of inhibition zone in this paper, using pathogens and probiotics commonly colonizing in the intestinal as indicators. The crude extract with antibacterial activity was further detected by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry ( HPLC-ESI-MS/MS) . The results of antibacterial assay displayed that the chloroform extract and n-butanol extract the corresponding culture media had not obvious inhibition activity against the tested strains. The crude lipopetide extact exhibited markedly antagonistic activity against Escherichia coli, Shigella flexneri and Salmo-nella enterica et cetera intestinal pathogenic bacteria and showed weak inhibitory effect on the probiotic Bulgar-ian lactobacillus, but had no effect on Streptococcus thermophilus. HPLC-ESI-MS/MS analysis data revealed that the active components in the crude lipopetide extact were C14 to C17 Mycosubtilin homologus. From all re-sults, it can be concluded that Mycosubtilin is produced and is responsible for the antibacterial activity of Bacil-lus subtilis strain N2-10.关键词
枯草芽孢杆菌/脂肽类物质/抑菌作用/高效液相色谱-电喷雾串联质谱/抗霉枯草菌素Key words
Bacillus subtilis/lipopeptides/antibacterial activity/HPLC-ESI-MS/MS/mycosubtilin分类
农业科技引用本文复制引用
李红亚,李术娜,王树香,王全,李文,朱宝成..益生芽孢杆菌脂肽类抑菌成分的高效液相色谱-电喷雾串联质谱分析[J].动物营养学报,2017,29(4):1191-1197,7.基金项目
河北省科技支撑计划项目(12226605) （12226605）
河北省高等学校科学技术研究项目(2011232) （2011232）
