动物营养学报2017，Vol.29Issue(4)：1205-1217,13.DOI:10.3969/j.issn.1006-267x.2017.04.016
体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱
Optimization of Non-Starch Polysaccharide Enzymes of Diets for Growing Pigs Using in Vitro Method
摘要
Abstract
Aimed to resolve the problem of massive work and poor comparability for screening non-starch poly-saccharides ( NSP) enzymes by animal experiment, the study was conducted to optimize the NSP enzymes combination of corn-soybean meal diet and corn-miscellaneous meal diet for growing pigs using an in vitro method. The first step was to determine the dose-response of the NSP enzymes supplemental level on the in vitro dry matter digestibility ( IVDMD) of diets using one-way randomized experimental design. The NSP en-zymes including cellulase, xylanase,β-glucanase,β-mannanase,α-galactosidase and pectinase were added in-to corn-soybean meal diet and corn-miscellaneous meal diet, and to analysis the effects of NSP enzymes on the IVDMD of diets. The second step was to screen the optimum enzyme combinations of 6 kinds NSP enzymes u-sing quadratic orthogonal rotation combination design. The results showed as follows: 1) quadratic effects of NSP enzymes on the IVDMD of diets for growing pig were observed. 2) The α-galactosidase had a greatest effect in all NSP enzymes of corn-soybean meal diet and increased the IVDMD by 1.28%; whereas xylanase increased the IVDMD of corn-miscellaneous meal diet by 1.95%. 3) The optimum enzyme combination in the corn-soybean meal diet was cellulase 533. 6 U/kg, xylanase 9983. 7 U/kg, β-glucanase 1014. 4 U/kg, β-mannanase 4080.6 U/kg,α-galactosidase 251.6 U/kg and pectinase 107.3 U/kg. The optimum enzyme com-bination in the corn-miscellaneous meal diet was cellulase 960.0 U/kg, xylanase 17177.6 U/kg, β-glucanase 405.8 U/kg, β-mannanase 19023.2 U/kg, α-galactosidase 307.2 U/kg and pectinase 96.9 U/kg. 4) The IVDMD of corn-soybean meal diet was increased by 3.26% and the IVDMD of the corn-miscellaneous meal di-et was also increased 3.75% using the optimum NSP enzymes combination. In conclusion, the combination of the 6 NSP enzymes can improve the IVDMD of corn-soybean meal diet and corn-miscellaneous meal diet for growing pig diets more effectively.关键词
非淀粉多糖酶/生长猪/饲粮/体外干物质消化率/优化Key words
NSP enzyme/pig/diet/in vitro dry matter digestibility/optimization分类
农业科技引用本文复制引用
高理想,陈亮,崔世贵,谢月华,张宏福..体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱[J].动物营养学报,2017,29(4):1205-1217,13.基金项目
国家科技支撑计划项目课题(2012BAD39B01) （2012BAD39B01）
中国农业科学院科技创新工程(ASTIP-IAS07) （ASTIP-IAS07）
产学研联合创新资金(BY2013054) （BY2013054）
苏北科技发展计划(BN2014034) （BN2014034）
