动物营养学报2017，Vol.29Issue(4)：1283-1293,11.DOI:10.3969/j.issn.1006-267x.2017.04.025
香蕉茎叶粉固态发酵条件优化及鹅对其养分利用率的研究
Study on Optimal Solid State Fermentation Conditions of Banana Stems and Leaves Powder and Its Nutrient Utilization for Geese
摘要
Abstract
This study was conducted to use the solid state fermentation technology to improve nutrient composi-tion of banana stems and leaves and its nutrient utilization for geese. Choosing Aspergillus oryzae and Candida utilis as fermentation strains to investigate the influences of 5 factors [ single strain fermentation, addition of (NH4)2SO4, proportion of Aspergillus oryzae and Candida utilis, mixed bacteria liquid inoculation] on the protein content of banana stems and leaves powder after fermentation. Using orthogonal design to find out the optimal combination of fermentation temperature, fermentation time and substrate moisture. The results showed that using Aspergillus oryzae or Candida utilis as single fermentation strain could significantly increase the crude protein content of fermented banana stems and leaves powder ( P<0. 05 or P<0. 01 ) . Fermentation with (NH4)2SO4 addition could significantly increase true protein content of fermented banana stems and leaves powder ( P<0.05 or P<0.01) , especially 2% addition. The net increase in protein treated with the proportion of Aspergillus oryzae and Candida utilis by 2:1 significantly higher than that of the proportion of 1:1, 1:3 and 3:2 ( P<0.05 or P<0.01) . The results of orthogonal test showed that the best fermentation condition were 4%inoculation (the proportion of Aspergillus oryzae and Candida utilis by 2:1) and 2% (NH4)2SO4 addition, and fermenting in 50% substrate moisture, 30 ℃ for 4 days. The crude protein content of banana stems and leaves increased by 33.82% after this fermentation process. The amino acid analysis showed that there were 15 kinds of amino acids increased differently beside lysine and arginine. The metabolic test results of Magang geese showed that the crude protein utilization of banana stems and leaves powder after fermentation significant-ly increased by 52.66% comparing with the banana stems and leaves powder without fermentation ( P<0.01) , additionally, the energy and metabolic energy utilization were increased slightly as well. In conclusion, the nu-tritional value of banana stems and leaves powder is improved after this fermentation process, and also promote the digestion and absorption of nutrient of geese.关键词
香蕉茎叶粉/固态发酵/蛋白质/养分利用率/马冈鹅/米曲霉/产朊假丝酵母Key words
banana stems and leaves powder/solid state fermentation/protein/nutrient utilization rate/Ma-gang geese/Aspergillus oryzae/Candida utilis分类
农业科技引用本文复制引用
王增煌,王文策,翟双双,谢强,左鑫,杨琳..香蕉茎叶粉固态发酵条件优化及鹅对其养分利用率的研究[J].动物营养学报,2017,29(4):1283-1293,11.基金项目
公益性行业科研专项(201303143) （201303143）
