动物营养学报2017，Vol.29Issue(4)：1335-1342,8.DOI:10.3969/j.issn.1006-267x.2017.04.031
脐带间充质干细胞通过类胰岛素样生长因子-Ⅰ介导的Janus激酶/信号转导及转录活化因子信号通路抑制奶牛乳腺上皮细胞凋亡的体外研究
An in Vitro Study of Umbilical Cord Mesenchymal Stem Cells Inhibite Bovine Mammary Gland Epithelial Cells Apoptosis by Insulin Like Growth Factor-Ⅰ Mediated Janus Kinase/Signal Transducer and Activator of Transcription Signaling Pathway
摘要
Abstract
This experiment was conducted to explore whether Janus kinase ( JAK)/signal transducer and acti-vator of transcription ( STAT ) signaling pathway was involved in regulation of umbilical cord mesenchymal stem cells ( UC-MSCs) by insulin like growth factor-Ⅰ ( IGF-Ⅰ) inhibited bovine mammary gland epithelial cells ( BMECs) apoptosis. UC-MSCs and BMECs were co-cultured by TranswellTM double chamber, BMECs cultured alone as control, cells was treated with insulin like growth factor-Ⅰ receptor ( IGF-ⅠR ) inhibitor AG1024 and signal blocking agent AG490. After 24 h, the relative expression abundances of B-cell lympho-ma/leukemia-2 ( Bcl-2) , Bcl-associated x protein ( Bax) , cysteine aspartic acid specific protease ( Caspase-3) were detected by real-time quantitative PCR ( RT-qPCR) , and flow cytometry was adopted to detect apoptosis changes of cells. The results showed as follows:the apoptosis rate of BMECs in UC-MSCs and BMECs co-cul-ture group was significantly lower than that in other groups ( P<0.01);compared with BMECs group, the rel-ative expression abundance of Bcl-2 gene in UC-MSCs and BMECs co-culture group was significantly increased ( P<0.01) , while the relative expression abundances of Caspase-3 and Bax genes were significantly decreased ( P<0.05 or P<0.01) . Treated by AG1024 or/and AG490, the apoptosis rate of single cultured BMSCs and co-cultured BMSCs with UC-MSCs was raised, and the relative expression abundances of Bax and Caspase-3 genes were up-regulated, while the relative expression abundance of Bcl-2 gene was down-regulated, and they were statistically significant ( P<0.05 or P<0.01) . In conclusion, UC-MSCs can regulate the expression of ap-optosis relation genes in BMECs by IGF-Ⅰ mediated JAK/STAT signaling pathway, and reduce the apoptosis rate of BMECs.关键词
脐带间充质干细胞/乳腺上皮细胞/共培养/IGF-Ⅰ/JAK/STAT/凋亡Key words
umbilical cord mesenchymal stem cells/mammary epithelial cells/co-culture/IGF-Ⅰ/JAK/STAT/apoptosis分类
农业科技引用本文复制引用
赵艳坤,邵伟,雒诚龙,武开乐,余雄..脐带间充质干细胞通过类胰岛素样生长因子-Ⅰ介导的Janus激酶/信号转导及转录活化因子信号通路抑制奶牛乳腺上皮细胞凋亡的体外研究[J].动物营养学报,2017,29(4):1335-1342,8.基金项目
国家奶产业体系(CARS-37) （CARS-37）
2015年国家自然科学基金项目(31560645) （31560645）
新疆农业大学畜牧学博士后工作站 （）
新疆维吾尔自治区高等学校科研计划项目(XJEDU2013S17) （XJEDU2013S17）
2013年度新疆研究生科研创新项目(xjau-2013-yjsky-XJGRI2013113) （xjau-2013-yjsky-XJGRI2013113）
新疆肉乳用草食动物营养实验室开放课题 （）
