动物营养学报2017，Vol.29Issue(4)：1350-1358,9.DOI:10.3969/j.issn.1006-267x.2017.04.033
谷氨酰胺对脂多糖诱导的断奶仔猪氧化应激的影响
Effects of Glutamine on Oxidative Stress of Weaned Piglets Challenged by Lipopolysaccharide
摘要
Abstract
This study was conducted to evaluate the effects of glutamine ( GLN) on oxidative stress of weaned piglets based on the oxidative stress model by Escherichia coli lipopolysaccharide ( LPS) . Twenty-four 28-day-old healthy crossbred ( Duroc×Landrace×Largewhite) weaned piglets were randomly assigned into three groups with eight replicates per group and one piglet per replicate. The piglets in control group and stress group were fed the basal diets, and the others in GLN group were fed the basal diet supplemented with 1% GLN. The ex-periment lasted for 30 days. At days 22, 25, 28 and 30 of the experiment, the piglets in stress group and GLN group were injected intraperitoneally with 100 μg LPS per kg body weight, whereas piglets in control group were injected intraperitoneally with the same dosage of sterile saline. At day 30, blood samples were collected by precaval vein and the intestinal tract samples were collected after slaughter for detection of the oxidative stress related indexes. The results showed as follows:1) serum antioxidant capacity indexes had no significant differences among all groups before LPS challenge ( P>0.05) . After LPS challenge, the content of malondial-dehyde ( MDA) in serum in stress group was significantly higher than that in control group ( P<0.05) , and the content of MDA and the activity of superoxide dismutase ( SOD ) in serum in GLN group were significantly lower than those in stress group and control group (P<0.05). 2) After LPS challenge, the relative gene ex-pression levels of catalase ( CAT) and Cu-Zn superoxide dismutase ( CuZuSOD) in duodenum mucosa in GLN group were significantly higher than those in stress group (P<0.05). The relative gene expression levels of CAT, manganese superoxide dismutase ( MnSOD) , glutathione peroxidase 1 ( GPX1) and glutathione peroxi-dase 4 ( GPX4) in jejunum mucosa in GLN group were significantly higher than those in control group and stress group (P<0.05), and the relative gene expression levels of GPX4 in control group was significantly higher than that in stress group ( P<0.05) . The relative gene expression level of CAT in ileum mucosa in GLN group and stress group was significantly lower than that in control group ( P<0.05) , and the relative gene ex-pression level of GPX4 was significantly higher than that in control group ( P<0.05) . The relative gene expres-sion level of MnSOD in ileum mucosa in GLN group was significantly higher than that in control group and stress group ( P<0.05) , and the relative gene expression level of CuZnSOD was significantly higher than that in control group ( P<0.05) . The results suggest that GLN can alleviate oxidative stress of weaned piglets chal-lenged by LPS to some extent, thereby provide a theoretical basis for reducing oxidative stress in actual produc-tion.关键词
谷氨酰胺/断奶仔猪/脂多糖/氧化应激/抗氧化能力Key words
glutamine/weaned piglets/lipopolysaccharide/oxidative stress/antioxidant capacity分类
农业科技引用本文复制引用
李欢,黄牛,何流琴,田军权,伍小松,姚康..谷氨酰胺对脂多糖诱导的断奶仔猪氧化应激的影响[J].动物营养学报,2017,29(4):1350-1358,9.基金项目
国家973项目专题(2013CB127301) （2013CB127301）
中科院"百人计划"项目 （）
国家自然科学基金面上项目(31472107) （31472107）
