动物营养学报2017，Vol.29Issue(4)：1359-1366,8.DOI:10.3969/j.issn.1006-267x.2017.04.034
牡蛎多糖锌配合物对脂多糖刺激断奶仔猪生长性能和肠道健康的影响
Effects of Oyster Polysaccharide-Zinc Complex on Growth Performance and Intestinal Health of Weaned Piglets Induced by Lipopolysaccharide
摘要
Abstract
This study was conducted to investigate the effects of oyster polysaccharide ( OPS)-zinc complex ( Zn-OPS) on growth performance of weaned piglets, and serum antioxidant capacity and anti-inflammatory factor content and disaccharidase activity in jejunum mucosa of immunized pigs with lipopolysaccharide (LPS). A total of thirty piglets of (28±1) days of age were randomly allocated into five groups named blank control ( group Ⅰ) , immunological stress control ( group Ⅱ) and low OPS ( group Ⅲ) , medium OPS ( group Ⅳ) and high OPS ( group Ⅴ) with six replicates per group and one pig in each replicate according to the principle of similar weight. Piglets in groups Ⅰ and Ⅱ were fed basal diets, and in groups Ⅲ, Ⅳ and Ⅴwere fed basal diets supplemented with 1000, 3000 and 5000 mg/kg Zn-OPS, respectively. The piglets were injected intraperitoneally with a dose of LPS ( 100 μg/kg BW ) except in group Ⅰ which were injected with the same dose of normal saline after 30 days of feeding experiment. And three hours after injection, the serum samples were collected to measure antioxidant capacity, and the jejunum mucosa was collected for measuring contents of interleukin ( IL)-2, IL-10 and tumor necrosis factor-α ( TNF-α) and disaccharidase activity by en-zyme linked immunosorbent assay. The results showed as follows:1) compare with groupsⅠandⅡ, average daily gain of weaned piglets in groupsⅣandⅤwas significantly increased ( P<0.05) , and the ratio of feed to gain in groups Ⅲ,ⅣandⅤwas decreased in some tendency ( P>0.05) . 2) Compare with groupⅠ, the ser-um total superoxide dismutase activity in groups Ⅲ, Ⅳ and Ⅴ was significantly increased (P<0.05). And compare with groupⅡ, the serum glutathione peroxidase activity in groupsⅢ,ⅣandⅤwas significantly in-creased ( P<0.05) , while the serum malondialdehyde content was significantly decreased ( P<0.05) . 3) Com-pare with group Ⅰ, the contents of IL-2 and IL-10 of jejunum mucosa in groups Ⅲ, Ⅳ and Ⅴ were signifi-cantly increased ( P<0.05) , and IL-10 content in group Ⅳ was significantly higher than that in group Ⅱ ( P<0.05) . Compare with group Ⅱ, TNF-α content of jejunum mucosa in groups Ⅲ and Ⅳ was significantly de-creased ( P<0.05) . 4) Compare with group Ⅱ, the activities of sucrose, maltase and lactase of jejunum mu-cosa in groups Ⅲ, Ⅳ and Ⅴ were significantly increased ( P<0.05) . It is concluded that dietary Zn-OPS can improve the serum antioxidant capacity and immune tolerance of piglets, increase the jejunum mucosal disac-charidase activity, which can relieve the immune stress. The effect of Zn-OPS supplemented with 3000 mg/kg is the best.关键词
牡蛎多糖锌配合物/断奶仔猪/生长性能/抗氧化能力/炎性细胞因子/二糖酶Key words
Zn-OPS/weaned piglets/growth performance/antioxidant capacity/inflammatory cytokines/disaccharidase分类
农业科技引用本文复制引用
马茂涛,林少伟,殷光文,黄志坚..牡蛎多糖锌配合物对脂多糖刺激断奶仔猪生长性能和肠道健康的影响[J].动物营养学报,2017,29(4):1359-1366,8.基金项目
福建省科技厅引导项目(2015N0017) （2015N0017）
国家自然科学基金——青年基金项目(31502058) （31502058）
校科技发展资金项目(KF2015017) （KF2015017）
