福建农业学报2017，Vol.32Issue(2)：107-110,4.DOI:10.19303/j.issn.1008-0384.2017.02.001
鸭疫里默氏菌铁依赖抑制子基因的原核表达
Prokaryotic Expression of RaDtxR Gene of Riemerella anatipestifer
摘要
Abstract
Based on the previously obtained characterization on the RaDtxR gene of Riemerella anatipestifer, upstream and downstream primers were designed with BamHI and Xho I restriction enzyme sites added at the 5'-teriminal.These quence of the RaDtxR gene was amplified and cloned into the prokaryotic expression vector pGEX-4T-1 to generate recombinant plasmids pGEX-4T-1-RaDtxR.Then, the expression conditions were optimized by IPTG induced by SDS-PAGE, and the biological activity identified by Western blot.SDS-PAGE and Western blot confirmed that the protein with a molecular weight approximating 51 ku was well expressed with a significant biological activity.Therefore, the highly efficient expression ofthe RaDtxR protein would provide a basis for further study associated with the specificgene.关键词
鸭疫里默氏菌/铁依赖抑制子基因/原核表达Key words
Riemerella anatipestifer/RaDtxR gene/prokaryotic expression分类
农业科技引用本文复制引用
万春和,程龙飞,陈红梅,傅光华,傅秋玲,施少华,陈翠腾,刘荣昌,黄瑜..鸭疫里默氏菌铁依赖抑制子基因的原核表达[J].福建农业学报,2017,32(2):107-110,4.基金项目
国家水禽产业体系项目(CARS-43) （CARS-43）
福建省畜禽疫病防控技术重大研发平台(2014N2003) （2014N2003）
福建省农业科学院"青年科技英才百人计划"专项(YC2015-12) （YC2015-12）
福建省优良蛋鸭品种与设施化养殖创新产业化工程项目(2014-2016) （2014-2016）
