福建农业学报2017，Vol.32Issue(2)：138-143,6.DOI:10.19303/j.issn.1008-0384.2017.02.007
利用ISSR标记分析36份番石榴种质资源的亲缘关系
Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers
摘要
Abstract
Using ISSR molecular markers,PCR products of 36 guava germplasms were purified by polyacrylamide gel electrophoresis and photographed the images for a UPGMA analysis to establish the genetic relationship.Out of 100 UBC series ISSR primers, 9 rich and stable polymorphic primers were selected and 165 bands amplified.Among the 165 bands, 129 were polymorphic rendering a polymorphism percentage of 78.18%.The amplified results could satisfactorily distinguish the 36 germplasms.The genetic similarity coefficient of all samples ranged from 0.70 to 0.93,averaging 0.815.At the coefficient of 0.75, the 36 germplasms were divided into 4 groups.The results were consistent with the morphological classification.Although the genetic basis for classification was weak,with the aid of ISSR molecular markers,clear identifications for the individual germplasms of the collection were obtained.关键词
番石榴/ISSR/亲缘关系Key words
guava/ISSR/genetic relationship分类
农业科技引用本文复制引用
宁琳,陈豪军,何江,杨祥燕,严霖,唐玉娟..利用ISSR标记分析36份番石榴种质资源的亲缘关系[J].福建农业学报,2017,32(2):138-143,6.基金项目
农业部热带作物种质资源保护项目(16RZZY-25) （16RZZY-25）
广西壮族自治区亚热带作物研究所基本科研业务费专项项目(桂热研201509) （桂热研201509）
