福建农业学报2017，Vol.32Issue(2)：149-154,6.DOI:10.19303/j.issn.1008-0384.2017.02.009
哈路比葡萄柚茎段的组培快速繁殖
Rapid Propagation for Hudson Foster Grapefruit Using Its Stems on Tissue Culture
摘要
Abstract
Stem segments of adult Hudson Foster grapefruit plants were used as explants to study the effect of hormones on the primary culture of axillary shoots, as well as that of hormones and media on the proliferation of clustered shoots.In addition, an attempt was made to refine the techniques on rooting, hardening and transplanting of the plantlets.The results showed that :(1) the optimized medium for primary culture was WPM+1.0 mg·L-1 6-BA/L+0.2 mg·L-1 NAA/L+40 g·L-1 to achieve up to 78.33% induction rate of axillary shoots;(2) the optimized medium for subculture multiplication was WPM+0.75 mg·L-1 6-BA+0.2 mg·L-1 NAA+40 g·L-1 sucrose to reach a multiplication coefficient of 4.93 with a shoot length of 2.62 cm;(3) the optimized medium for rooting was 1/2WPM+0.1 mg·L-1 NAA+1.0 mg·L-1 IBA+40 g·L-1sucrose+0.1% AC to obtain strong roots at a rooting rate of 63.33% with a plant height up to 3.03 cm;(4) the survival rate of transplanted plantlets after rooting was greater than 78%.关键词
葡萄柚/组培/初代诱导/增殖/生根Key words
grapefruit/tissue culture/primary culture/proliferation/rooting分类
农业科技引用本文复制引用
叶维雁,刘惠民,刘月,唐军荣,李贤忠,李文兵,夏赛..哈路比葡萄柚茎段的组培快速繁殖[J].福建农业学报,2017,32(2):149-154,6.基金项目
国家国际科技合作专项项目(2014DFA31060) （2014DFA31060）
