福建农业学报2017，Vol.32Issue(2)：155-160,6.DOI:10.19303/j.issn.1008-0384.2017.02.010
水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定
Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing
摘要
Abstract
Valid sampling is essential to obtain reliable transcripts of genetic information for biological studies.For RNA sequencing, the integrity, purity and concentration of total RNA as well as the cDNA libraries and transcriptome sequencingon two specimens of tea shoots sampled by using two methods were compared.One of the methods was hydroponic culture (SM1), and another,dry ice freezing (SM2).The results showed that both of them yielded RNA extractions with high integrity and purity without significant differences on the measured concentrations.They were well satisfied for the subsequent transcriptome sequencing, also capable of obtaining the equivalent cDNA libraries and RNA sequencing.However, SM1 was simpler and more convenient than SM2 in handling and operation, and particularly suitable for field sampling.Aside from being used on tea, SM1 could be applied on other crops in preparing samples for extracting RNA as well.关键词
茶树/转录组测序/RNA提取/水培枝条Key words
tea plant(Camellia sinensis)/RNA sequencing/RNA extraction/hydroponic culture分类
农业科技引用本文复制引用
高香凤,王让剑,刘丰静,李慧玲..水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定[J].福建农业学报,2017,32(2):155-160,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1012-3) （2014R1012-3）
