福建农业学报2017，Vol.32Issue(2)：161-166,6.DOI:10.19303/j.issn.1008-0384.2017.02.011
响应面法优化双孢蘑菇菌株W38液体菌种培养的研究
Optimization by Response Surface Methodology for Agaricus bisporus W38 Fermentation in Liquid Medium
摘要
Abstract
Based on the results obtained by a previous single factor test, medium formulation and conditions for the fermentation of Agaricus bisporus W38 were optimized by the Plackett-Burman experimental design and the central composite design with Design-Expert software.A regression equation was obtained using the response surface methodology.The optimized medium was formulated toconsistof 39.90 g·L-1, 13.60 g·L-1, K2HPO4 2.0 g·L-1, and MgSO4·7H2O 1.067 5 g·L-1.The fermentation was designed to be carried out with an initial pH of 6.5 and maintained at 24℃ with a constant shaking in flask at 180 r·min-1 for 7 d.A mycelial biomass 100 mL of 2.35 g, which was 3.05-foldof the original design, and an average mycelial growth rate improvement by 14.7% were obtained.关键词
双孢蘑菇/液体菌种/响应面/优化Key words
Agaricus bisporus/liquid fermentation/response surface methodology/optimization分类
农业科技引用本文复制引用
曾志恒,程翊,曾辉,杨雷,廖剑华,施肖堃,蔡志英,戴建清..响应面法优化双孢蘑菇菌株W38液体菌种培养的研究[J].福建农业学报,2017,32(2):161-166,6.基金项目
国家食用菌产业技术体系项目(CARS24) （CARS24）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1020-2、2017R1020-2) （2015R1020-2、2017R1020-2）
国家农业科技成果转化项目[闽财指(2014)969号] （2014）
