法医学杂志2017，Vol.33Issue(2)：168-170,3.DOI:10.3969/j.issn.1004-5619.2017.02.013
MPure-12全自动核酸纯化仪与Chelex-100法的比较
Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method
摘要
Abstract
Objective T o explore the forensic application value of M Pure-12 autom atic nucleic acid pu-rification (M Pure-12 M ethod) for D N A extraction by extracting and typing D N A from bloodstains and various kinds of biological sam ples w ith different D N A contents. Methods N ine types of biological sam-ples, such as bloodstains, sem en stains, and saliva w ere collected. D N A w ere extracted using M Pure-12 m ethod and C helex-100 m ethod, follow ed by PC R am plification and electrophoresis for obtaining ST R-profiles. Results T he sam ples such as hair root, chutty, butt, m uscular tissue, saliva stain, bloodstain and sem en stain w ere typed successfully by M Pure-12 m ethod. Partial alleles w ere lacked in the sam ples of saliva, and the genotyping of contact sw abs w as unsatisfactory. A dditional, all of the bloodstains (20μL , 15μL , 10μL , 5μL , 1μL ) show ed good typing results using C helex-100 m ethod. B ut the loss of alleles occurred in 1μL blood volum e by M Pure-12 m ethod. Conclusion M Pure-12 m ethod is suitable for D N A extraction of a certain concentration blood sam ples.C helex-100 m ethod m ay be better for the ex-traction of trace blood sam ples.T his instrum ent used in nucleic acid extraction has the advantages of sim plicity of operator, rapidity, high extraction efficiency, high rate of reportable ST R-profiles and low er m an-m ade pollution.关键词
法医遗传学/提取法/自动化,实验室/MPure-12全自动核酸纯化仪/Chelex-100法Key words
forensic genetics/extraction/autom ation/laboratory/M Pure-12 autom atic nucleic acid purifi-cation/C helex-100 m ethod分类
社会科学引用本文复制引用
盛翔,李敏,王亚丽,陈玉玲,林源,赵珍敏,阙庭志..MPure-12全自动核酸纯化仪与Chelex-100法的比较[J].法医学杂志,2017,33(2):168-170,3.基金项目
十三五国家重点研发计划项目(2016YFC0800703) （2016YFC0800703）
中央级科研院所公益资助项目(GY2014G-4,GY2017D-2) （GY2014G-4,GY2017D-2）
上海市法医学重点实验室资助项目(17DZ2273200) （17DZ2273200）
上海市司法鉴定专业技术服务平台资助项目(16DZ2290900) （16DZ2290900）
