南方农业学报2017，Vol.48Issue(4)：698-703,6.DOI:10.3969/j.issn.2095-1191.2017.04.022
七叶一枝花总黄酮提取工艺优化
Optimization of extraction technique for total flavonoids from Paris Polyphylla
摘要
Abstract
[Objective]In the present study, response surface methodology was applied to optimize the process condi-tions for the microwave-assisted extraction of total flavonoids from Paris Polyphylla, in order to improve the extraction yield of total flavonoids and provide technique support for actual production. [Method]Based on single factor experiments, three major parameters including alcohol volume fraction, microwave power and liquid-solid ratio were optimized using Box-Behnken certral composite design, in a bid to select the optimal process parameters for total flavonoids extraction from P. Polyphylla. [Result]The regression model based on extraction of total flavonoids from P. Polyphylla(Y), ethanol volume fraction(A), liquid-solid ratio(B) and microwave power(C) was as follows:Y=4.90+0.30A+0.51B+0.48C-0.19AB+0.44AC+0.24BC-0.85A2-0.83B2-0.55C2(R2=0.9734). The fitting degree was high. The three parameters and interaction between ethanol volume fraction and microwave power had extremely significant influence on total flavonoids extraction yield (P<0.01). The optimal extraction conditions were as follows: ethanol volume fraction 50%, liquid-solid ratio 17:1 and mi-crowave power 528 W. Under such conditions, the extraction yield was 5.28 mg/g. The relative error was 1.93% compared to the predicted yield (5.18 mg/g). [Conclusion]Parameters of microwave-assisted total flavonoids extraction from P. Poly-phylla optimized by response surface methodology are feasible, which can be applied to actual production.关键词
七叶一枝花/总黄酮/微波辅助提取/响应面法/工艺优化Key words
Paris Polyphylla/total flavonoids/microwave-assisted extraction/response surface methodology/pro-cess optimization分类
医药卫生引用本文复制引用
王永立,袁浩,樊淑华..七叶一枝花总黄酮提取工艺优化[J].南方农业学报,2017,48(4):698-703,6.基金项目
河南省科技计划项目(162102310587) （162102310587）
