南方农业学报2017，Vol.48Issue(4)：721-727,7.DOI:10.3969/j.issn.2095-1191.2017.04.026
罗氏沼虾TLRs基因表达差异分析
Differential expression of TLRs gene in Macrobrachium rosenbergii
摘要
Abstract
[Objective]By analyzing the differential expressions of three Toll-like receptor(TLRs) genes including Mr-Toll1, MrToll2, MrToll3 in different tissues of Macrobrachium rosenbergii and expression difference of gill at different times after infection with Vibrio alginolyticus, the present study provided reference for revealing immune function of M. rosen-bergii TLRs in fighting diseases. [Method]Real-time fluorescence quantitative RT-PCR was applied to detect relative ex-pressions of MrToll1, MrToll2 and MrToll3 in muscle, gill, hepatopancreas, heart, stomach, gut, hemolymph, nerve gan-glion, and eyestalk. The same method was also used to test the expression changes of TLRs gene after infected by V. algi-nolyticus. [Result]MrToll1, MrToll2 and MrToll3 genes were expressed in all of the tested tissues and there also existed differential expressions. MrToll1 and MrToll2 genes highly expressed in gill among these tissues while the relative expression of MrToll1 and MrToll2 in gill were as much as 19.26 times and 20.03 times as those in hepatopancreases respectively. The expression of MrToll3 gene was the highest in nerve ganglions, which was as 10.13 times as that in hepatopancreases. After infected by V. Alginolyticus, mortality rate of M. rosenbergii increased as time spent, and reached the peak at 72 post infection(64%). After 72 h post infection, no more mortality occurred. After infection with V. alginolyticus, the expression of MrToll1 gene rose first and then decreased, and reached the peak 24 h post infection. The expression of MrToll2 went through a down-up-down-up variation, and reached the maximum 24 h post infection. The expression of MrToll3 gene de-creased sharply and maintained at low level. [Conclusion]MrToll1 gene is major response gene for gill resisting bacterial in-fection, MrToll2 gene may be involved in other immune activities of gill, and regulation mechanism of MrToll3 is unknown.关键词
罗氏沼虾/Toll样受体(TLRs)/表达差异/溶藻弧菌/实时荧光定量PCRKey words
Macrobrachium rosenbergii/Toll-like receptor(TLRs)/differential expression/Vibrio alginolyticus/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
刘伟利,刘红,丁福江,戴习林..罗氏沼虾TLRs基因表达差异分析[J].南方农业学报,2017,48(4):721-727,7.基金项目
上海市科学技术委员会重点攻关项目(11391901400) （11391901400）
上海市科技兴农重点攻关项目(沪农科攻字[2010]第1-6号) （沪农科攻字[2010]第1-6号）
上海市教育委员会重点学科建设项目(J50701) （J50701）
