华北农学报2017，Vol.32Issue(2)：45-49,5.DOI:10.7668/hbnxb.2017.02.007
拟南芥抗灰霉病基因T1N6_22互作蛋白的筛选与分析
Screening and Analysis of Interacting Proteins of T1N6_22 Gene from Arabidopsis Against Botrytis cinerea
摘要
Abstract
Botrytis susceptible mutant named T1N6_22 was achieved from Arabidopsis mutant library.Through the method of TAIL-PCR,resistance-related gene T1N6_22 of Arabidopsis thaliana against Botrytis cinerea infection was cloned.In order to further clarify the regulation mechanism of T1N6_22 gene in Arabidopsis thaliana against Botrytis cinerea infection,vector pAS1/T1N6_22 of T1N6_22 gene was constructed.Taking pAS1/T1N6_22 fusion protein as bait,Arabidopsis cDNA library were screened by the method of yeast two-hybrid system.A total of 28 yeast clones were obtained by yeast two hybrid screening.The positive clones were identified by T1N6_22 gene specific primers and sequenced.Four interacting proteins(AT2G19480,AT1G06050,AT5G34780 and AT1G21400)were obtained by screening Arabidopsis cDNA library.Blast analysis showed that four interacting proteins encoded nucleosome assembly protein,function unknown protein,putative ketopantoate reductase,and oxidoreductase,respectively.These interacting proteins were related to process,biosynthesis of pantothenate,and plant metabolic process.These results would provide foundation for identifying interacting protein of T1N6_22 and clarifying the regulation mechanism of T1N6_22 gene in Arabidopsis resistance.关键词
拟南芥/T1N6_22/酵母双杂交/互作蛋白Key words
Arabidopsis/T1N6_22/Yeast two-hybrid/Interacting protein分类
生物科学引用本文复制引用
瓮巧云,黄聪聪,王娜,梁晨曦,刘高然,郝丛丛,邢继红,董金皋..拟南芥抗灰霉病基因T1N6_22互作蛋白的筛选与分析[J].华北农学报,2017,32(2):45-49,5.基金项目
河北省自然科学基金项目(C2014405010) （C2014405010）
