华北农学报2017，Vol.32Issue(2)：81-86,6.DOI:10.7668/hbnxb.2017.02.013
蒙古扁桃编码Ca2+结合蛋白基因的系统性鉴定和表达分析
Mongolian Almond Ca2+ Binding Protein Encoding Gene Systemic Identification and Expression Analysis
摘要
Abstract
Mongolian Almond(Prunus mongolica Maxim)belongs to Rosaceae,under national protection of second class,primarily distributes at the Northwestern Dessert Region of China and is tolerant to drought and poor soil.Calcium ion is the most important signaling molecule for plant sensing and resistance to environment stresses.In this study,based on transcriptome analysis of the leaf,we identified sixteen strings of full length transcripts,including four Ca2+ ATPase(ECA/ACA),three novel Ca2+ channel protein with several transmembrane domains(ERD),five Ca2+/H+antiporter(CAX),two Ca2+ dependent protein kinase(CPK)and two calmodulins(CAM).Their predicated structures are similar to those homologies in Arabidopsis.Four single genes from the ERD,CAX,CPK and CAM categories,respectively,had obvious expression in the leaves and roots,among which the ERD,CPK and CAM genes expression had been regulated by drought and salt at different extent.It indicated that the genes might modulate the plant′s environmental stress-tolerant ability.关键词
蒙古扁桃/转录组测序/钙结合蛋白/钙调素Key words
Mongolian almond/Transcriptome/Ca2+ binding protein/Calmodulin分类
农业科技引用本文复制引用
刘洋,铁英,杨佳,祁智,毛惠平..蒙古扁桃编码Ca2+结合蛋白基因的系统性鉴定和表达分析[J].华北农学报,2017,32(2):81-86,6.基金项目
内蒙古自治区科技重大专项(内财教 [2014]2020号) （内财教 [2014]2020号）
