华北农学报2017，Vol.32Issue(2)：96-103,8.DOI:10.7668/hbnxb.2017.02.015
柠条锦鸡儿CkLEA4-1基因的克隆及表达分析
Clone and Expression Analysis of CkLEA4-1 from Caragana korshinskii Kom.
摘要
Abstract
In order to research the biological function of the LEA protein,a gene encoding LEA protein from the suppression subtractive hybridization library of Caragana korshinskii Kom.was screened and cloned by using RT-PCR method.The open reading frame(ORF)of the gene length was 1 119 bp,encoded a protein of 373 amino acids,named CkLEA4-1.Based on sequence alignment and phylogenetic analysis,it was inferred that CkLEA4-1 belonged to group 5 late embryogenesis abundant protein(LEA).The expression of CkLEA4-1 under high salt and drought conditions were detected by Real-time quantitative PCR,the results showed that CkLEA4-1 could be induced at different levels,which suggested that the gene might be involved in stress response of Caragana korshinskii Kom..The over-expression vector pCanG-CkLEA4-1 of CkLEA4-1 was constructed successfully,and the transgenic homozygous lines were obtained,which provided materials for the further study of CkLEA4-1 gene function in Caragana korshinskii Kom..关键词
柠条锦鸡儿/LEA/基因克隆/表达分析Key words
Caragana korshinskii Kom./LEA/Gene cloning/Expression analysis分类
农业科技引用本文复制引用
滑璐玢,于秀敏,杨杞,王瑞刚,宝力德..柠条锦鸡儿CkLEA4-1基因的克隆及表达分析[J].华北农学报,2017,32(2):96-103,8.基金项目
国家自然科学基金项目(31560199 （）
31360169) （）
内蒙古自治区科技创新团队(201503004) （201503004）
内蒙古自治区草原英才团队 （）
