华北农学报2017，Vol.32Issue(2)：104-108,5.DOI:10.7668/hbnxb.2017.02.016
紫苏脂肪酸硫酯酶基因PfFatA生物信息学及其表达特性分析
Bioinformatics and Expression Characteristics Analysis of Fatty Acid Thioesterase Gene in Perilla frutescens
摘要
Abstract
In order to detect the function of fatty acid thioesterase (FatA) in fatty acid synthesis mechansim of perilla, many bioinformatics analytical methods were used to analyze the protein encoded by FatA gene.The result showed that full length of cDNA for PfFatA was 1 652 bp,open reading frame(ORF)was 1 119 bp,coding 372 amino acids.FatA was an unstable hydrophilic protein,without transmembrane region.FatA protein contained a conserved domain of Acyl-ACP_TE,belonging to the Hotdog fold super protein family.Multiple sequence alignment indicated that FatA had highly conserved triple peptide of AKL,SKV in the C-terminal.Phylogenetic tree analysis showed that Perilla frutescens had the closest genetic relationship with Salvia miltiorrhiza.The result of Real-time PCR showed PfFatA was expressed in roots,stems,leaves,flowers and seeds from different development period,especially in seeds formed in 10 days after flowering.These results of the current study provided a foundation for further illustrating the function and mechanism of PfFatA gene.关键词
紫苏/脂肪酸硫酯酶基因(FatA)/生物信息学/表达分析Key words
Perilla frutescens/Fatty acid thioesterase/Bioinformatics/Expression analysis分类
农业科技引用本文复制引用
李璐,梁倩,安茜,周雅莉,王计平..紫苏脂肪酸硫酯酶基因PfFatA生物信息学及其表达特性分析[J].华北农学报,2017,32(2):104-108,5.基金项目
国家自然科学基金项目(31201266) （31201266）
