华北农学报2017，Vol.32Issue(2)：109-116,8.DOI:10.7668/hbnxb.2017.02.017
甘蓝型油菜GPDH基因克隆及其生物信息学分析
Cloning and Bioinformatics Analysis of GPDH in Rapeseed
摘要
Abstract
To further study on GPDH of Brassica napus,through the collinearity analysis of the GPDH from Arabidopsis thaliana and Brassica napus:there are 4 copies of GPDH of A genome and C genome in the Brassica napus called as GSBRNA2T00132785001,GSBRNA2T00024258001,GSBRNA2T00058395001,GSBRN-A2T00019118001.Four cDNA were downloaded from GenBank,are named as:GPDH1,GPDH2,GPDH3 and GPDH4.Four pairs of primers were designed according to the sequence informations,and four nucleotide fragments were amplified from Xiangyou 15 full-length cDNA,designated as BnGPDH1,BnGPDH2,BnGPDH3,BnGPDH4.The result of nucleotide sequences and protein structure analysis by bioinformatics software and online system showed:Four CDS sequences length were 1 395,1 395,1 389,1 536 bp respectively.When BnGPDH4 is translated into the amino acid sequence,we found a stop codon in the middle of BnGPDH4.Which isn′t an active protein.The number of amino acid of three other BnGPDH are 464,464 and 462 respectively.Secondary structure are consistant of the alpha helix(42%),random coil(42%)and extended chain(15%).The transmembrane structure of the three proteins was uniform.Their conservative domains consist of GpsA,NADB_Rossmann superfamily and NAD_Gly3P_dh_C superfamily,which belong to the GPDH superfamily.Homology modeling analysis showed that they were of model of Glycerol-3-phosphate dehydrogenase and located in cytoplasm by subcellular localization analysis.In terms of sequence composition,phylogenic tree,physico-chemical property,secondary structure and tertiary structure and subcellular localization,they have the characteristics of GPDH superfamily.They should be the members of GPDH superfamily.关键词
GPDH/甘蓝型油菜/生物信息学分析Key words
GPDH/Brassica napus L./Bioinformatics analysis分类
生物科学引用本文复制引用
刘少锋,汪慧慧,邬克彬,熊兴华..甘蓝型油菜GPDH基因克隆及其生物信息学分析[J].华北农学报,2017,32(2):109-116,8.基金项目
国家"973"计划项目(2015CB150200) （2015CB150200）
湖南省科技计划项目(2014FJ1006-3) （2014FJ1006-3）
