华北农学报2017，Vol.32Issue(2)：117-123,7.DOI:10.7668/hbnxb.2017.02.018
牦牛皮肤中调控毛色基因表达定量和黑色素细胞组织学分析
Quantitative the Control Colour Gene Expression and the Melanin Cell Histologic Analysis in the Yak Skin
摘要
Abstract
The test was to through the molecular level and histological level shed light the different mechanism of coat color in yak.The experiment adopted the Real-time fluorescent quantitive PCR technique to detect the skin tissue determines the main effect of pigment formation pathway genes:gray mouse gene(agouti signaling protein gene (ASIP),Agouti),small eye malformation associated transcription factor (microphthalmia-associated transcription factor,MITF),melanin cortisol receptor 1 gene(melanocortin receptor 1,MC1R) and tyrosine enzyme gene(tyrosinase gene,TYR) expression.The results showed that the MC1R expression quantity in black the color was higher than the white color;MITF expression quantity in the black coat with white color difference was obvious;ASIP expression quantity in white color and black color was not different;TYR expression quantity in Tianzhu white coat was Datong pure black coat 2.87 times,this result was opposite the colored coat with high expression result,the TYR expression quantity of yak melanin synthesis effect remained to be further research.Test made by paraffin section method,using HE and toluidine blue staining.Mammalian hair by hair follicle growth and development of derivatives,the melanothore mainly distributed in the epidermis and hair follicles.Through microscope found the yak hair follicle was made up of wool sheath,hair papilla and connective tissue around the group formed outside the bulb.A lot of melanothores were found in skin and around hair follicels in Tianzhu white yak,and found that there was a small amount of melanin granules.Melanin granules were found in hair follicle and epidermis melanothore in Datong yak.The melanin content near the hair dermal papilla was higher than other parts around the hair follicle in black coat hair follicle.Datong yak hair follicle group was more serried than Tianzhu yak hair follicle group,and the diameter of the hair follicle was longer than Tianzhu yak,with thicker hair.The melanin cell around the hair follicle of Datong yak was denser than Tianzhu yak,and the nucleus was larger,and more obvious.关键词
牦牛/毛色基因/定量表达/石蜡切片/黑色素Key words
Yak/Genes about colour/RT-PCR/Paraffin section/Melanin分类
生物科学引用本文复制引用
高泽成,梁春年,吴晓云,李明娜,张志飞,阎萍..牦牛皮肤中调控毛色基因表达定量和黑色素细胞组织学分析[J].华北农学报,2017,32(2):117-123,7.基金项目
科技部科技支撑计划项目(2012BAD13B05) （2012BAD13B05）
现代农业(肉牛牦牛)产业技术体系(CARS-38) （肉牛牦牛）
