解放军医学杂志2017，Vol.42Issue(4)：285-289,5.DOI:10.11855/j.issn.0577-7402.2017.04.03
氧化应激在中暑大鼠急性肝损伤中的作用及机制研究
The role of oxidative stress in the acute liver injury of rats with heat stroke and its mechanism
摘要
Abstract
Objective To investigate the role of oxidative stress in acute liver injury in a heat stroke model of conscious rats,and to explore its underlying mechanism.Methods Thirty-two rats were randomly (by using a random number table) assigned into a sham-heated control group (Sham group,n=8),a sham-heated group treated with NAC (Sham-NAC group,n=8),a heat stroke group (HS group,n=8) and a heat stoke group treated with NAC (HS-NAC,n=8).Rats were prepared with pre-warm chamber to initiate heat stoke.The change of rectum temperature (Tr),heart rate (HR) and systolic blood pressure (SBP) were monitored,and the time point of HS onset was recorded.Rats were sacrificed 12h after HS onset.ALT,serum TBIL,IL-6,IL-1β,TNF-α,MDA,T-SOD and GSH in the liver homogenates were measured.Liver tissues were harvested for determining the concentration of reactive oxygen species (ROS),neutrophil infiltration and the histological changes.Results During HS onset,no significant differences were observed in Tr,HR,SBP and heat exposure time between HS group and HS-NAC group (P＞0.05).However,the survival time was significantly longer in HS-NAC group than in HS group (P=0.039).12 hours after HS onset,the concentrations of ROS and MDA in the liver homogenates were significantly higher in HS group than in the other groups (P=0.000),while the concentrations of T-SOD and GSH were much lower than in the other groups (P=0.000).The serum concentrations of ALT and TBIL were significantly higher in HS group than in the other groups (P=0.000).Compare with HS group,the pathological injury was alleviated in HS-NAC group (P=0.000).The neutrophil infiltration level and the concentrations of IL-6,IL-1 β and TNF-α in liver tissue were significantly higher in HS group than in HS-NAC group (P=0.000).Conclusion Oxidative stress may play an important role in the pathogenesis of HS liver injury through its cytotoxic effect and by inducing inflammatory responses.关键词
中暑/氧化应激/肝损伤Key words
heat stroke/oxidative stress/liver injury分类
医药卫生引用本文复制引用
耿焱,彭娜,童华生,潘志国,刘云松,马强,苏磊..氧化应激在中暑大鼠急性肝损伤中的作用及机制研究[J].解放军医学杂志,2017,42(4):285-289,5.基金项目
国家自然科学基金(81671896,81471839) （81671896,81471839）
中国博士后科学基金(2012M512181) （2012M512181）
广西省自然科学基金(2016GXNSFAA380313) （2016GXNSFAA380313）
广东省自然科学基金(2016A030310288,2014A030313601) （2016A030310288,2014A030313601）
广东省科技计划项目(2013B031800010)This work was supported by the National Natural Science Foundation of China (81671896,81471839),China Postdoctoral Science Foundation (2012M512181),Province Natural Science Foundation of Guangxi (2016GXNSFA380313),Natural Science Foundation of Guangdong Province (2016A030310288,2014A030313601),and Science and Technology Project of Guangdong Province (2013B031800010) （2013B031800010）
