解放军医学杂志2017，Vol.42Issue(4)：290-294,5.DOI:10.11855/j.issn.0577-7402.2017.04.04
乌司他丁对重症中暑小鼠心功能损害的保护作用及机制研究
Protective effects of ulinastatin on cardiac dysfunction in mice with heat stroke and its mechanism
摘要
Abstract
Objective To examine the effects ofulinastatin (UTI) on cardiac dysfunction in mice with heat stroke and its possible mechanism.Methods 20 mice were divided into four groups randomly:room temperature plus normal saline (Sham+NS),room temperature plus UTI (Sham+UTI),heat stress plus normal saline (HS+NS),heat stress plus UTI (HS+UTI),5 each.105U/kg UTI was delivered by intraperitoneal injection before the onset of the heat stress.Room temperature groups were housed at room temperature (23.0 ± 0.5 ℃),while heat stress groups were kept in an incubator at 36.5 ± 0.5 ℃ and humidity of 65.0％ ± 2.0％.The rectal temperature (Tr) reaching 42℃ was taken as severe heat stroke,and the time in two heat stress groups was recorded.The mice were transferred to the room temperature (23.0 ± 0.5 ℃) for natural cooling after the heat stroke onset.6 hours after the treatment,cardiac output (CO) was ultrasonographically detected,the myocardium was separated for histopathological examination and the expression of total p38 and phosphorylated p38 (p-p38) was determined by Western blotting.Results The time to reach 42℃ in HS+UTI group was significantly prolonged (P=0.044).Compared with the Sham+NS group,the CO in HS+NS and HS+UTI group decreased significantly (P=0.017),and the score of myocardial inflammation (P＜0.001) and p-p38/p38 ratio (P＜0.001) increased.The CO was significantly higher in HS+UTI group than in HS+NS group (P=0.030),and the score of myocardial inflammation (P＜0.001) and p-p38/p38 ratio (P=0.001) were significantly lower.Conclusion Ulinastatin might improve the cardiac function in mice with heat stroke by decreasing the p-p38 and alleviating the inflammation response.关键词
中暑/乌司他丁/心功能/炎症Key words
heat stroke/ulinastatin/cardiac function/inflammation分类
医药卫生引用本文复制引用
吉晶晶,李俊,李慧,孙学刚,苏磊,刘志锋..乌司他丁对重症中暑小鼠心功能损害的保护作用及机制研究[J].解放军医学杂志,2017,42(4):290-294,5.基金项目
国家自然科学基金(81571940) （81571940）
军队“十二五”医学科研基金重点项目(BWS12J108) （BWS12J108）
广东省自然科学基金团队项目(s2013030013217) （s2013030013217）
广州市科技计划项目(201504281714528)This work was supported by the National Natural Science Foundation of China (81571940),the Key Project of the "Twelfth Five-Year Plan"for Medical Research of PLA (BWS12J108),the Project Team of Natural Science Foundation of Guangdong Province (s2013030013217),and the Guangzhou Science and Technology Planning Project (201504281714528) （201504281714528）
