解放军医学杂志2017，Vol.42Issue(4)：295-300,6.DOI:10.11855/j.issn.0577-7402.2017.04.05
热休克蛋白70对中暑大鼠急性肺损伤的保护作用及机制研究
Protective effects of heat shock protein 70 on the acute lung injury of rats with heat stroke and its mechanism
摘要
Abstract
Objective To investigate the protective effect of heat shock protein (HSP) 70 on the acute lung injury (ALI) of rats with heat stroke.Methods Sixty four rats were randomly (by employing a random number table) assigned into a sham-heated group (Sham group),heat stress group (HS group),and HS plus gluttamine treatment group (HS+GLN group) and HS plus quercet in treatment group (HS+QU group),16 each.All rats were housed in a artificial climate chamber,with the rats in the sham groups exposed to a temperature of 23 ℃ and humidity of 55％ ± 5％,while the rats of HS,HS+GLN and HS+QU groups to an ambient temperature of 39 ℃ and humidity of 65％.During heat stress or sham heating,rectal temperature (Tr),systolic blood pressure (SBP) and pulse rate (PR) were monitored to observe the difference in heat stress response among the groups.The time point at which the SBP started to drop from the peak level was taken as the point of HS onset.At the onset of HS,heat exposure was terminated,then the rats were immediately removed from the chamber,and returned to room temperature.The rats were scarified 0h and 6h after HS onset respectively.After bronchoalveolar lavage fluid (BALF) was collected,the lungs of all animals were harvested for pathological examination of lung injury.The concentrations of IL-1β,TNF-α and IL-6 in BALF and HSP70 in lung homogenate were measured by using an enzyme linked immunosorbent assay kit.Results Compared with HS and HS+QU groups,the rats in HS+GLN group required significantly greater heat load to induce HS (P＜0.001),and had longer survival time span after HS onset.Compared with Sham group,the concentration of HSP70 in lung homogenate in HS group increased in a time-dependent manner (P＜0.001).In comparison with HS group,the concentration of HSP70 in lung homogenate from HS+GLN group was significantly elevated at each time point (P＜0.001),while the treatment with QU significantly inhibited the expression of HSP70 (P＜0.001).The concentration of IL-1β,TNF-α and IL-6 in BALF significantly decreased in HS+GLN group compared with those in HS group and HS+QU group (P＜0.001).The pathological results showed that the lung injury was milder in HS+GLN group,while the opposite in HS+QU group.Conclusion HSP70 could protect HS rats against ALI by enhancing their thermo-tolerance and inhibiting inflammatory response.关键词
中暑/HSP70热休克蛋白质类/肺损伤Key words
heat stroke/HSP70 heat shock proteins/lung injury分类
医药卫生引用本文复制引用
耿焱,彭娜,童华生,潘志国,刘云松,马强,苏磊..热休克蛋白70对中暑大鼠急性肺损伤的保护作用及机制研究[J].解放军医学杂志,2017,42(4):295-300,6.基金项目
国家自然科学基金(81671896,81471839) （81671896,81471839）
中国博士后科学基金(2012M512181) （2012M512181）
广西省自然科学基金(2016GXNSFAA380313) （2016GXNSFAA380313）
广东省自然科学基金(2016A030310288,2014A030313601) （2016A030310288,2014A030313601）
广东省科技计划项目(2013B031800010)This work was supported by the National Natural Science Foundation of China (81671896,81471839),China Postdoctoral Science Foundation (2012M512181),Natural Science Foundation of Guangxi Province (2016GXNSFA380313),Natural Science Foundation of Guangdong Province (2016A030310288,2014A030313601),and Science and Technology Project of Guangdong Province (2013B031800010) （2013B031800010）
