解放军医学杂志2017，Vol.42Issue(4)：307-313,7.DOI:10.11855/j.issn.0577-7402.2017.04.07
食管癌细胞来源的外泌体对肿瘤细胞迁移及侵袭能力的影响及机制研究
Effects of esophageal cancer cell-derived exosomes on cancer cell migration and invasion and its mechanism research
摘要
Abstract
Objective To investigate the biological effects of exosomes secreted by KYSE410 cells on migration and invasion of KYSE410,KYSE510,YES2 cells and the possible mechanisms underlying the phenotype change.Methods The exosomes were isolated from the conditional supernatant of esophageal cancer cell line KYSE410 by ultracentrifugation.The morphology of exosomes was observed by transmission electron microscopy (TEM).Western blotting was used to detect the protein markers of exosomes.The uptaken of fluorescence-labeled KYSE410 exosomes by KYSE410,KYSE510 and YES2 was also recorded under confocal microscopy.Migration and invasion ability of the three esophageal carcinoma cell lines and the effects of exosomes from KYSE410 on migration and invasion of KYSE410,KYSE510 and YES2 cells were analyzed by Transwell chamber,respectively.The alteration of Wnt/β-catenin and PI3K/Akt pathway-related proteins were detected by Western blotting.Results The membrane structure of KYSE410 derived exosomes could be observed with its diameter ranged between 30-100nm.The invasion and migration ability of three esophageal cancer cells are KYSE410＞ KYSE510＞ YES2.KYSE410 exosomes promoted the migration and invasion of KYSE410,KYSE510 and YES2 cells.Conclusions Concentrated exosomes derived from the highly migratory and invasive esophageal cancer cell line KYSE410 promoted the migration and invasion potentials of itself and esophageal cancer cell lines KYSE510 and YES2,which possibly exerted the effects by activating Wnt/β-catenin and PI3K/Akt signaling pathways.关键词
食管癌/外泌体/肿瘤迁移/肿瘤侵袭Key words
esophageal cancer/exosome/cancer migration/cancer invasion分类
医药卫生引用本文复制引用
林锋,王海娟,李春晓,李慧,王婷,南鹏,钱海利,詹启敏..食管癌细胞来源的外泌体对肿瘤细胞迁移及侵袭能力的影响及机制研究[J].解放军医学杂志,2017,42(4):307-313,7.基金项目
国家自然科学基金(81572842、81672459) （81572842、81672459）
中国医学科学院医学与健康科技创新工程项目(2016-12M-1-001)This work was supported by the National Natural Science Foundation of China (81572842,81672459) and CAMS Innovation Fund for Medical Sciences (2016-I2M-1-001) （2016-12M-1-001）
