兰州大学学报（医学版）2017，Vol.43Issue(2)：7-11,5.DOI:10.13885/j.issn.1000-2812.2017.02.002
重离子辐照对HepG2细胞迁移、侵袭和黏附的影响及其机制
Effects of heavy ion irradiation on migration, invasion and adhesion of HepG2 cells and its mechanism
摘要
Abstract
Objective To investigate the effects of 12C6+ heavy ion irradiation on migration,invasion and adhesion of human hepatocellular carcinoma HepG2 cells and its related mechanisms.Methods The logarithmic HepG2 cells were irradiated by 0,1,2,4 Gy of 12C6+ heavy ion ray and then the migration and invasion were determined by Transwell method,the adhesion ability was detected by MTS method (inner salt method),and the matrix metalloproteinases-2 (MMP-2) and MMP-9 protein expression was detected by Western blot.Results After 12C6+ heavy ion rays irradiation,compared with the 0 Gy group,with the increase of radiation dose,HepG2 cell migration ability decreased significantly,the invasion ability also decreased (P ＜ 0.05);HepG2 cell adhesion ability enhanced (P ＜ 0.05);the amount of MMP-2 and MMP-9 expression in HepG2 cells were down-regulated (P ＜ 0.05).Conclusion 12C6+ heavy ion irradiation can enhance the adhesion ability and inhibit the migration and invasion ability of HepG2 cells,which may be related to the down-regulation of MMP-2 and MMP-9 expression.关键词
重离子/HepG2细胞/迁移/侵袭/黏附/基质金属蛋白酶Key words
heavy ion/HepG2 cell/migration/invasion/adhesion/matrix metalloproteinases分类
医药卫生引用本文复制引用
张建刚,陈逸轩,娄丽丽..重离子辐照对HepG2细胞迁移、侵袭和黏附的影响及其机制[J].兰州大学学报（医学版）,2017,43(2):7-11,5.基金项目
国家级大学生创新创业训练计划项目(201410730136) （201410730136）
敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX1213-014) （DHYX1213-014）
