兰州大学学报（医学版）2017，Vol.43Issue(2)：37-42,6.DOI:10.13885/j.issn.1000-2812.2017.02.007
中药脉平片质量控制标准的提高
Improving quality standard of Maipingpian
高进贤 1张秀娟 2呼延丽 1高栓生 1朱菊花 1葛斌1
作者信息
- 1. 甘肃省人民医院药剂科,甘肃兰州730000
- 2. 甘肃省人民医院质控科,甘肃兰州730000
- 折叠
摘要
Abstract
Objective To establish a quality standard for Maipingpian,a Chinese medicine.Methods Thinlayer chromatography (TLC) was applied to the identification of Ginkgo biloba leaves (EGb),and TLC of emodin and physcion was improved.Total flavonol glycosides determined by high performance liquid chromatography (HPLC) was used with a photo-diode array detector.Results TLC of Ginkgolide A,EGb,emodin and physcion had distinct characteristic spots of separation and there was no interference in the negative comparison.The linear range of quercetin was between 0.029-0.588 μg (r=0.999 7);Kaempferol was between 0.062--0.411 μg (r=0.999 8);Isorhamnetin was between 0.057-0.566 μg (r=0.999 8).The mean recovery of quercetin was 97.22％,RSD=l.28％;Kaempferol was 98.15％,RSD=1.40％;Isorharrnetin was 97.16％ and RSD=l.47％.Conclusion TLC and HPLC are accurate,reliable and simple in operation,which can be used for the quality control of Maipingpian.关键词
脉平片/银杏叶提取物/总黄酮醇苷/薄层色谱法/高效液相色谱法Key words
Maipingpian/Ginkgo biloba leaves/total flavonol glycosides/thin-layer chromatography/high performance liquid chromatography分类
医药卫生引用本文复制引用
高进贤,张秀娟,呼延丽,高栓生,朱菊花,葛斌..中药脉平片质量控制标准的提高[J].兰州大学学报（医学版）,2017,43(2):37-42,6.
