老年医学与保健2017，Vol.23Issue(2)：118-121,4.
肝细胞生长因子对紫外线诱导皮肤成纤维细胞产生活性氧的影响
Effect of Hepatocyte Growth Factor on Reactive Oxygen Species in UVA-induced Skin Fibroblasts
摘要
Abstract
Objective To explore the effect of hepatocyte growth factor (HGF) on reactive oxygen species (ROS) in UVA-induced skin fibroblasts.Methods The effect of HGF of different dosage on the activity of fibroblasts (FBs) was detected by cck-8 assay;after HGF pretreatment for 2 hours,ROS test probe was loaded and oxidative stress of FBs was induced by UVB irradiation in vitro;the expression of ROS was measured by fluorescence and enzyme-labeling instrument respectively.Results 5ng/ml HGF had no obvious effect on FBs proliferation;the expression of ROS increased greatly in UVB-induced oxidative stress (P＜0.01);5ng/ml HGF reduced the ROS expression caused by UVB induction (P＜0.05).Conclusions HGF can reduce ROS expression in FBs induced by UVB irradiation and decrease oxidative stress,it can be taken as a new anti-photoaging measure.关键词
HGF/光老化/成纤维细胞/ROSKey words
hepatocyte growth factor (HGF)/photoaging/fibroblasts (FBs)/reactive oxygen species (ROS)引用本文复制引用
杨清建,刘天一,毕波,贾传龙,陈亮,卢勇舟,周轶群,杨平,郭妤,朱晶晶..肝细胞生长因子对紫外线诱导皮肤成纤维细胞产生活性氧的影响[J].老年医学与保健,2017,23(2):118-121,4.基金项目
国家自然科学基金资助项目(81272125 （）
81301642) （）
上海市卫生系统优秀学科带头人培养计划资助项目(XBR2011033) （XBR2011033）
863项目(SS2014AA020705) （SS2014AA020705）
