南京医科大学学报（自然科学版）2017，Vol.37Issue(4)：423-427,5.DOI:10.7655/NYDXBNS20170408
过表达Mxi1对人胃癌SGC-7901细胞增殖和凋亡的影响
Effects of overexpression of Mxi1 on cell proliferation and apoptosis of human gastric cancer SGC-7901 cells
摘要
Abstract
Objective:To construct a lentiviral vector containing Mxi1 gene and observe the effect of Mxi1 on proliferation and apoptosis of SGC-7901 cells.Methods:The Mxi1 gene was cloned into lentiviral expression vector by recombining DNA technology.The recombinant plasmid was identified by restriction enzyme digestion and gene sequencing,and then cotransfected 293T cells with the auxiliary packaging components plasmids to obtain recombinant lentivirus containing Mxi1 gene.SGC-7901 cells were infected with the recombinant lentivirus,and the infection efficiency was observed under fluorescence microscope.Expressions of Mxi1,cyclinB1 and caspase-8 gene were identified by RT-PCR and expression of Mxi1 protein was identified by Western blot.The proliferation and apoptosis of SGC-7901 cells were examined by CCK-8 and flow cytometry,respectively.Results:The Mxi1 gene was successfully cloned to lentiviral,expressions of Mxi1 gene and protein were confirmed by RT-PCR and Western blot.Mxi1-overexpressed SGC7901 cells expressed less cyclinB1 and more caspase-8 compared to blank control cells and empty vector-overexpressed cells confinned by RT-PCR.CCK-8 and flow cytometry experimental results showed that the proliferation ability of Mxi1-overexpressed SGC-7901 cells was deteriorated significantly compared to blank control cells and empty vector-overexpressed cells,and the apoptosis rate of Mxi1-overexpressed cells was higher than that of blank control cells and empty vector-overexpressed cells.Conclusion:The Mxi1 recombinant lentiviral vector had been successfully constructed and effectively transfected SGC-7901 cells.Mxi1 could inhibit the proliferation and promote the apoptosis of SGC-7901 cells.关键词
Max结合蛋白1/人胃癌SGC-7901细胞/增殖/凋亡/慢病毒载体Key words
Max interacting protein 1/SGC-7901 cell/proliferation/apoptosis/lentiviral vector分类
医药卫生引用本文复制引用
胡圳圳,蒋秀琴,许金金,郭文文,郑大同..过表达Mxi1对人胃癌SGC-7901细胞增殖和凋亡的影响[J].南京医科大学学报（自然科学版）,2017,37(4):423-427,5.基金项目
国家自然科学基金(81301822) （81301822）
南京医科大学科技发展基金(2012NJMU088) （2012NJMU088）
