中国海洋大学学报(自然科学版)2017,Vol.47Issue(6):53-60,8.DOI:10.16441/j.cnki.hdxb.20160250
熊本牡蛎(♀)×葡萄牙牡蛎(♂)杂交子代的营养成分和脂肪代谢相关基因表达分析
Determination of Nutritive Components and Expression Analysis of Lipid Metabolism Related Genes of Hybrid of Kumamoto Oyster (♀) and Portuguese Oyster (♂)
摘要
Abstract
The basic components, amino acids, fatty acids and the expression of three genes related to the lipid metabolism (PPARα, INSIG, SREBP) of one year old oyster Crassostrea.sikamea, C.angulata and their hybrid were determined and analyzed, respectively in this study.The results showed that the content of lipid in hybrid was (13.66±1.99)%, significantly higher than that of C.sikamea (7.56±1.17)% and C.angulata (5.94±1.88)%.PUFAω3/PUFAω6 ratio was 4.18±0.62 in hybrid, higher than that in C.sikamea (3.86±0.01) and C.angulata (3.14±0.07).The content of DHA in hybrid (16.73±3.35)% was slightly by not significantly higher than that in self-propagated parents.The EPA content of hybrid was between that of C.sikamea and C.angulata.The expression of lipid genes (PPARα, INSIG, SREBP) in some edible tissues of oysters coincided well with lipid content.The results provided a basis thatnutritional heterosis existed in hybrid.关键词
熊本牡蛎/葡萄牙牡蛎/杂交牡蛎/营养成分/基因表达Key words
Crassostrea sikamea/Crassostrea angulata/hybrid oyster/nutritional component/gene expression分类
农业科技引用本文复制引用
闫路路,王昭萍,苏家齐,闫喜武,于瑞海..熊本牡蛎(♀)×葡萄牙牡蛎(♂)杂交子代的营养成分和脂肪代谢相关基因表达分析[J].中国海洋大学学报(自然科学版),2017,47(6):53-60,8.基金项目
国家自然科学基金项目(31172403)资助 Supported by the National Natural Science Foundation of China(31172403) (31172403)